Abbott Laboratories Corporation

Wlodzimierz Mandecki

Timothy J Bolling

John C Russell

Suresh M Desai

Dominique P Bridon

Sushil G Devare

James M Casey

Grant A Krafft

Catherine A Brennan

Elaine Marie Brate

Steven C March

Keeve D Jaffe

Vincent T Yue

This invention relates to genetically engineered enzymes, their ligand conjugates, their manufacture, and their use in qualitative or quantitative assays. A hybrid enzyme, such as an AP-epitope, has a foreign amino acid moiety (an epitope) inserted near the active site of the starting AP enzyme. The foreign amino acid moiety binds with an analyte, and, as a consequence of this binding, the enzymatic activity of the hybrid enzyme, AP-epitope, is modified. The changes in the enzymatic activity are dependent upon the presence, or the amount, of the analyte. In another embodiment, the hybrid enzyme consists of a cysteine introduced near the active site of an AP to give a hybrid enzyme. The cysteine on the hybrid enzyme serves as a point of conjugation of a ligand, such as theophylline, ferritin, thyroxine, or digoxigenin, to form the hybrid enzyme-ligand conjugate. The ligand binds with an antibody, an analyte or a binding molecule to an analyte and as a result of this binding, the enzymatic activity of the hybrid enzyme-ligand conjugate is modified or modulated.

Genetically engineered enzymes and their conjugates for diagnostic assays

Improved methods for detecting antibodies in test samples. The improvement comprises uses CKS-fusion proteins specific for the antibodies in assays such as screening assays, competitive assays, confirmatory assays and immunodot assays. Test kits which contain these CKS-fusion proteins useful in such assays also are provided.

Methods for using CKS fusion proteins

Disclosed is a method of producing fusion proteins, particularly HCV fusion proteins, wherein one part of the fusion protein is formed from the bacterial protein CKS.

CKS method of HCV protein synthesis

Disclosed is a method of producing fusion proteins wherein one part of the fusion protein is formed from the bacterial protein CKS.

CKS method of protein synthesis

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Libertyville, IL, United States of America

Wlodzimierz Mandecki