University of Utah Research Foundation

University of Washington

Wesley Sundquist

Neil P King

David Baker

Marc J Lajoie

Jacob Bale

Elizabeth Gray

Daniel Stetson

Yang Hsia

Joerg Votteler

Gabriel Butterfield

Eiji Morita

Jun Arii

Jörg Philip Votteler

Disclosed herein are modified capsid proteins comprising a capsid forming protein, a membrane binding element and an ESCRT-recruiting element, wherein at least one of the membrane binding element and the ESCRT-recruiting element is heterologous to the capsid forming protein. Disclosed are capsids comprising a plurality of modified capsid proteins. Disclosed are multimeric assemblies comprising a plurality of capsids within a membrane. Also disclosed are modified non-enveloped viruses comprising a capsid wherein the capsid comprises a plurality of modified capsid proteins, wherein the plurality of modified capsid proteins comprise a capsid forming protein, a membrane binding element and an ESCRT-recruiting element, wherein at least one of the membrane binding element and the ESCRT-recruiting element is heterologous to the modified capsid protein, wherein the capsid forming protein is a capsid forming protein of a non-enveloped virus.

Engineering virus-like nanocarriers for biomolecule delivery

The application discloses multimeric assemblies including multiple oligomeric substructures, where each oligomeric substructure includes multiple proteins that self-interact around at least one axis of rotational symmetry, where each protein includes one or more polypeptide-polypeptide interface ('O interface'); and one or more polypeptide domain that is capable of effecting membrane scission and release of an enveloped multimeric assembly from a cell by recruiting the ESCRT machinery to the site of budding by binding to one or more proteins in the eukaryotic ESCRT complex ('L domain'); and where the multimeric assembly includes one or more subunits comprising one or more polypeptide domain that is capable of interacting with a lipid bilayer (&#x201c;M domain&#x201d;), as well as membrane-enveloped versions of the multimeric assemblies.

Polypeptide assemblies and methods for the production thereof

A non-natural modified CMV promoter is provided. Such a promoter can include a promoter nucleotide sequence that is at least 80% homologous to a sequence selected from the group consisting of SEQ ID 01, SEQ ID 02, SEQ ID 03, SEQ ID 04, SEQ ID 05, SEQ ID 06, SEQ ID 07, and compliments thereof.

Attenuated protein expression vectors and associated methods

Growing community of inventors

Salt Lake City, UT, United States of America

Wesley Sundquist