Food Industry Research and Development Institute

Food Industry Research and Development

Wen-Shen Chu

Chiou-Yen Wen

Shie-Jea Lin

Min Tseng

Su-Fang Chung

Mei-Li Chao

Jaw-Shiow Chu

Yun-Huey Lee

Guey-Yuh Liou

Jen-Tao Chen

Yi-Shin Lin

Chang-Hsiesh Liu

Li-Lin Chen

Chang-Cheng Huang

Wen-Haw Hsu

Ming-Chu Chen

Wen-Hwei Hsu

Yi-Jang Lee

Gwo-Jen Liao

Proteins isolated fromhaving prolyl oligopeptidase activity, nucleic acids encoding the protein and methods for producing and using the protein, wherein SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:9 and SEQ ID NO:10 must be contained therein to at least 60% similarity. The proteins and nucleic acids have improved heat stability and perform more favorably in vivo having optimum activity conditions around 40 degrees centigrade and around pH 7, and can therefore be used in medicaments for the treatment of celiac disease caused by proline abundant gluten or other applications.

Protein having prolyl oligopeptidase activity, nucleic acid encoding same and method for producing and using same

A protein of amino acid sequence SEQ ID NO:6 isolated from. The protein is stable at 30 to 37 degrees centigrade and has an optimal prolyl oligopeptidase activity at about 45 degree centigrade and at about pH 7. The present invention also relates to a medicament comprising such protein for treating celiac disease caused by proline abundant gluten.

Protein having prolyl oligopeptidase activity, and composition containing the same

A DNA molecule encoding transglutaminase, a transglutaminase, an expression vector containing the DNA molecule, and a cell containing the expression vector.

Transglutaminase

An isolated strain of thespecies that converts glucose to erythritol with a conversion rate of at least about 45% is disclosed, as is a method of producing erythritol from such a strain.

Erythritol-producing moniliella strains

The invention provides a DNA molecule encoding transglutaminase of the encoded transglutaminase and the use of the transglutaminase in industrial process.

Transglutaminase gene of Streptoverticillium ladakanum and the transglutaminase encoded therefrom

An isolated strain of the Moniliella species that converts glucose to erythritol with a conversion rate of at least about 45% is disclosed, as is a method of producing erythritol from such a strain.

Erythritol&mdash;producing Moniliella strains

A method of producing erythritol. The method includes growing in a culture an isolated yeast strain that possesses certain unique characteristics and is capable of converting glucose to erythritol in a glucose-containing medium, and purifying erythritol from the culture.

Erythritol-producing yeast strains

A yeast strain capable of converting glucose to erythritol, said strain having the following identifying characteristics: an absence of motile spores; septate mycelia; asexual reproduction; an absence of reniform cells; conidia optionally formed on short denticles but not on elongate stalks; an absence of ballistoconidia; non-monopolar budding on a broad base; acropetal chains of blastoconidia; dark brown, thick-walled chlamydospores; an ability to assimilate sucrose, glycerol and maltose; an inability to assimilate lactose; an inability to ferment galactose; an ability to grow in a vitamin-free medium; and an ability to grow at 25&deg; C. to 36&deg; C.

This invention relates to the isolation of thermophilic phytase-producing microorganisms, method for producing phytase using such microorganisms, phytase obtained therefrom, and usage of the phytase to hydrolyze phytic acid or phytate. In particular, this invention relates to phytase-producing microorganisms, which belong to Streptomyces sp., Pseudonocardia sp. or Microbispora sp., and which produce phytase available for recovery in an efficient and practical manner.

Phytase-producing bacteria, phytase and production method of phytase

Phytase-producing bacteria

This invention relates to a D-amino acid oxidase of the genus Rhodosporidium and a gene encoding it.

Rhodosporidium D-amino acid oxidase

A method of screening for a given enzyme in colony-forming cells using a substrate of the enzyme that has low solubility. Use of such a method leads to the discovery of glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase of Pseudomonas nitroreducens. Also disclosed is a method of obtaining GL-7-ACA acylase from Pseudomonas cells.

Screening and characterization of glutaryl-7-aminocephalosporanic acid

A method of converting cephalosporin C to glutaryl-7-aminocephalosporanic acid. The method including obtaining a cell preparation from a microorganism which produces D-amino acid oxidase, the preparation being a cell-free extract or a suspension of permeated cells; adding a D-.alpha.-amino acid to the preparation; after the adding step, heating the preparation at 50.degree.-75.degree. C. for 5-60 minutes; and incubating cephalosporin C in the preparation. Also disclosed are a method of converting cephalosporin C to glutaryl-7-aminocephalosporanic acid in the absence of exogenous H.sub.2 O.sub.2, and a method of screening for a D-amino acid oxidase-producing microorganism.

Growing community of inventors

Hsinchu, Taiwan

Wen-Shen Chu