Jiangnan University

Weizhu Zeng

Jian Chen

Jingwen Zhou

Guocheng Du

Meng Ning

Qiuju Zhang

Xiaomei Cao

Jun Fang

Li Liu

Fang Fang

Song Liu

Jie Chen

Jurong Ping

Song Gao

Qian Zhang

Xiaolin Liang

Zhaojun Wang

Disclosed is recombinantfor producing L-tyrosine and application thereof, and belongs to the technical fields of genetic engineering and bioengineering. According to the present disclosure, genes aroP and tyrP are knocked out, expresses the endogenous gene yddG of, then heterologously expresses fpk from, expresses the endogenous genes ppsA and tktA of, and then expresses aroGand tyrA. Knocking out tyrR, trpE and pheA, so that the synthesis flux of L-tyrosine is increased. Finally, an endogenous gene poxB is knocked out to realize stable fermentation performance at high glucose concentration.

Recombinantfor producing l-tyrosine and application thereof

The present disclosure provides an automatic production line for manufacturing and processing plant protein meat, and belongs to the field of application of food equipment. The automatic production line for manufacturing and processing the plant protein meat comprises: an extrusion-expansion machine, plant protein meat raw material blocks, a bearing plate, a first manipulator, a storage rack, a first conveying device, a second manipulator, a second conveying device, a main console, a third conveying device, a shredding device, a seasoning adding and mixing device, a third manipulator, a recycling rack, a storage cabinet and a secondary console. The shredding device of the present disclosure shreds the plant protein meat raw material blocks by cooperative up-down staggered motion of double rows of clips, the plant protein meat raw material blocks in any shape can be shredded, and breakage of the raw material shreds in the shredding process can be avoided effectively; and by the seasoning adding and mixing device of the present disclosure, the shredded plant protein meat raw material blocks are added with seasonings and mixed with the seasonings. The present disclosure is simple in structure, facilitates manufacturing of the plant protein meat, and can efficiently produce and process the plant protein meat.

Automatic production line for manufacturing and processing plant protein meat

The disclosure discloses construction of recombinant Saccharomyces cerevisiae for synthesizing carminic acid and application thereof and belongs to the technical field of genetic engineering and bioengineering. The disclosure obtains recombinantCA-B2 capable of synthesizing carminic acid by heterologously expressing cyclase Zhul, aromatase ZhuJ, OKS of Octaketide synthase 1, C-glucosyltransferase UGT2, monooxygenase aptC and 4'-phosphopantetheinyl transferase npgA in. The recombinantcan be used for synthesizing carminic acid by taking self-synthesized acetyl-CoA and malonyl-CoA as a precursor. On this basis, OKS, cyclase, aromatase, C-glucosyltransferase and monooxygenase relevant to carminic acid are integrated to a high copy site, which can remarkably improve the yield of carminic acid. The yield of carminic acid can be increased to 2664.6 &#x3bc;g/L by optimizing fermentation conditions, and the fermentation time is shortened significantly. Therefore, the recombinantplays an important role in the fields of cosmetics, textiles and food.

Construction of recombinantfor synthesizing carminic acid and application thereof

The present disclosure relates to a method for improving the yield and production intensity of() sorbose, and belongs to the technical field of fermentation engineering. By knocking out genes related to formation of D-sorbitol or L-sorbose metabolic by-products in, the formation of the by-products is reduced, and the efficiency of transforming D-sorbitol into L-sorbose is improved, thereby improving the yield and production intensity of L-sorbose. A recombinant strain-11 constructed by the present disclosure, compared with a control strain, has an L-sorbose transformation rate of 96.12%, which is 4.47% higher than that of a wild strain, has a production intensity of 14 g/L&#xb7;h, which is 14.7% higher than that of the wild strain, and has a fructose by-product content of only 5.6 g/L, which is 45.6% lower than that of the wild strain.

Method for improving yield and production intensity ofsorbose

The present disclosure provides a method for extracting alpha-ketoglutarate and pyruvate simultaneously from microbial fermentation broth or enzyme transformation solution, which is related to the technical field of biological separation and extraction. The method comprises the following steps: centrifuging the microbial fermentation broth or enzymatic conversion solution containing &#x3b1;-KG and PA to remove the cells and other visible solids; removing the macromolecular impurities by ultrafiltration; evaporating and concentrating under reduced pressure conditions; extracting with the water-insoluble extraction after acidification; separating crude crystals of &#x3b1;-KG and crude liquid of PA by evaporation crystallization method (if concentration of PA is great higher than that of &#x3b1;-KG, crystallization separation should be conducted after distilling partial pure pyruvate); washing the crude crystal of &#x3b1;-KG with water-insoluble organic solvent as ethyl acetate or butyl acetate, drying and crushing to obtain qualified &#x3b1;-KG; distilling to gain qualified PA product applying high vacuum distillation (or molecular distillation).

Method for extracting alpha-ketoglutarate and pyruvate simultaneously from microbial fermentation broth or enzymatic conversion solution

The invention provides a high-throughput screening system based on multi-manipulators, and belongs to the field of biotechnology and detection equipment. A high-throughput screening system based on multi-manipulators, comprises of the first manipulator, sampler, pipette, plate washer, microplate reader, the second manipulator, centrifuge, deep-well plate library, waste shallow-well plate barrel, shallow-well plate library, waste needle plate barrel, needle library, waste deep-well plate barrel, collection box. The present invention is a combination of microbiology and mechanics. The aim of the invention is to realize the automation and intelligentization of the high throughput screening experiment, effectively improve the experimental accuracy, reliability and efficiency. It contributes to the development of high throughput screening technology for microorganisms and drugs.

High-throughput screening system based on multi-manipulators

The present invention provides a production-line-type high-throughput screening system, which relates to the field of biotechnology and testing equipment. The system comprises of four manipulators, three parallel conveyor belts with fixed slots, 2-DOF slipway and fixed fixtures, 96-channel pipetting system, coloring device, oscillating mixing device, microplate reader, well plates loading platform and well plates recycling platform. Manual operation takes five minutes to detect one 96-well plate, while this system can handle 20 96-well plates per minute. It expands the number of screening targets, making the screening process more clearly and concisely and liberating manual labor. The system makes effective contributions to the development of microbial breeding technology.

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Wuxi, China

Weizhu Zeng