Applied Biosystems, LLC

Other

Applera Corporation

Université De La Méditerranée

Bi, Wanli

Wanli Bi

Khairuzzaman Bashar Mullah

Zhaochun Ma

Kenneth J Livak

Will Bloch

Emmanuel Beillard

Jacques Van Dongen

Jean Gabert

The present invention provides a method for amplification of a target nucleic acid sequence or signal, wherein an amplification reaction mixture is used which contains at least one reversibly modified oligonucleotide having a non-hydroxyl group 3' end which can be converted into a hydroxyl 3&#x2032; end upon exposure to a chemical and/or irradiation and/or a range of temperature. The present invention also provides a reversibly modified oligonucleotide as described above, and a nucleic acid amplification reaction mixture and kit comprising such an oligonucleotide.

Nucleic acid amplification using a reversibly modified oligonucleotide

Disclosed, among other things, are primers containing certain modified nucleobases in the 3' terminal region of the primers that provide reduced formation of primer-dimers during amplification reactions, and various methods of use thereof.

Modified oligonucleotides  and applications thereof

Modified oligonucleotides and applications thereof

Disclosed are methods for detecting or quantifying one or more target polynucleotide sequences in a sample. In one aspect, a sample is contacted with first and second probe pair that are capable of hybridizing to a selected target sequence and a corresponding complementary sequence, respectively. Probe cleavage and ligation results in the formation of ligation products which can be generated in an exponential fashion when the target sequence and/or complement are present in the sample. In another embodiment, a single probe pair can be used to form ligation product in a linear fashion from a complementary template. Reagents and kits are also disclosed.

Polynucleotide sequence assay

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San Ramon, CA, United States of America

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