Princeton University

Wai-Leung Ng

Bonnie L Bassler

Martin F Semmelhack

Lark J Perez

Douglas A Higgins

Jianping Cong

Megan Eileen Bolitho

Kristina M Kraml

Using a whole-cell high-throughput screen, eleven molecules were identified that activatequorum sensing (QS). Eight molecules are receptor agonists and three molecules are antagonists of LuxO, the central NtrC-type response regulator that controls the globalQS cascade. Pro-QS molecules are used for the development of novel anti-infectives.

Broad spectrum pro-quorum-sensing molecules as inhibitors of virulence in vibrios

A bacterial autoinducer, CAI-1, was purified and its structure identified. Methods for synthesis of the autoinducer and its analogues were elucidated. Methods of using the autoinducer or its analogues for treating bacterial pathogenicity and bio film formation are described. Methods for prevention and treatment of cholera are described. Synthetic (S)-3-hydroxytridecan-4-one functions as well as natural CAI-1 in repressing production of the virulence factor toxin co-regulated pilus (TCP). Strategies are described to manipulate bacterial quorum sensing in the clinical arena.

Identification of bacterial autoinducer and use in treating bacterial pathogenicity

Growing community of inventors

Lawrenceville, NJ, United States of America

Wai-Leung Ng