Ambergen, Inc.

Adeptrix Corp.

Other

Vladislav B Bergo

Kenneth J Rothschild

Mark J Lim

Sadanand Gite

Alex Garvin

Alex M Garvin

Bead-based assays for measuring protein biomarkers of proteolytic activity in biological systems are disclosed. In an embodiment, an assay involves incubating a sample containing multiple fragments of a naturally occurring protein with a bead array and subsequently analyzing individual reactive sites of the bead array by mass spectrometry.

Bead-based assays for protein analysis

Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.

Global proteomic screening of random bead arrays using mass spectrometry imaging

Bead-based analytical assays suitable for detecting changes in the abundance of target analytes in biological samples are disclosed. In an embodiment, an assay involves incubating a sample with one or several beads that are capable of binding several distinct analytes in an amount sufficient for detection by mass spectrometry from a single bead.

Multiplexed bead-based analytical assays

Bead arrays suitable for analysis by mass spectrometry are disclosed. In an embodiment, a bead array includes multiple reactive sites, each of the reactive sites being capable of binding multiple distinct target analytes.

Multiplexed bead arrays for proteomics

Microarray compositions suitable for analysis by one or several spectrographic methods are disclosed. In an embodiment, a microarray composition includes a three-dimensional solid support and a plurality of reactive microbeads positioned on the solid support in spatially distinct and addressable locations.

Microarray compositions and methods of their use

Eluting analytes from bead arrays

Global Proteomic screening of random bead arrays using mass spectrometry imaging

This invention relates to the detection and analysis by mass spec of nascent proteins, and in particular truncated proteins, translated within cellular or cell-free translation systems. N-terminal and C-terminal epitopes introduced into these nascent proteins permit rapid and efficient isolation, as well as mass difference.

Growing community of inventors

Boston, MA, United States of America

Vladislav B Bergo