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Mirus Bio Corporation

Roche Madison Inc.

Vladimir M Subbotin

Jon Asher Wolff

James E Hagstrom

Vladimir G Budker

Julia Hegge

Sean D Monahan

Hans Herweijer

A process is described for transfecting nucleic acids into cells in vivo by mixing transfection reagents and the nucleic acids immediately prior to injection. The process comprises introducing the transfection reagents and the nucleic acids into a mixing chamber by a first portal for introducing the transfection reagents, a second portal for introducing the nucleic acids where the transfection reagents and nucleic acids are mixed together to form particles and pushing the particles through an injection portal into a mammal in less than 1 second after introduction thereby transfecting cells in the mammal.

Process for mixing nucleic acids with transfection reagents for delivery

Disclosed is a system for providing in vivo delivery of molecules or complexes to extravascular mammalian cells using an intravascular administration route. The molecules or complexes are inserted in an injection solution into a mammalian vasculature. Insertion of the injection solution at an appropriate rate transiently increases the volume of extravascular fluid in the tissue thereby facilitating delivery of the molecule to the cell.

Delivery of molecules and complexes to mammalian cells in vivo

A process is described for the delivery of a therapeutic polynucleotide to limb muscle tissue suffering from or potentially suffering from ischemia. The polynucleotide is inserted into a mammalian limb vessel such as an artery. Delivery efficiency and distribution is enhanced by combining injection of a solution containing the polynucleotide with the use of an externally applied cuff.

Devices and processes for distribution of genetic material to mammalian limb

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Madison, WI, United States of America

Vladimir M Subbotin