The General Hospital Corporation

Dana-farber-cancer Institute Inc.

The Broad Institute, Inc.

Ute E Burkhardt

Nir Hacohen

Edward F Fritsch

Catherine Ju-Ying Wu

The present disclosure relates to methods for profiling subject specific and personalized T cell receptor (TCR) repertoires using a single-cell sequencing method. More particularly, disclosed are methods for determining binding of T cell receptors to subject specific neoantigens. In addition, the techniques herein may identify the antigenic targets of T cell receptors in the context of tumor neoantigens. Moreover, the present disclosure enables the discovery of T cell targets in numerous diseases, with implications for understanding the basic mechanisms of the mammalian immune response and for developing antigen-specific diagnostic markers and therapies. Finally, cloned TCRs can be used to formulate personalized immunotherapies for those inflicted with a disease, such as cancer.

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Belmont, MA, United States of America

Ute E Burkhardt