Council of Scientific and Industrial Research

Upasana Singh

Dhiman Sarkar

Swati Pramod Joshi

Roshan Rajan Kulkarni

Ketaki Dilip Shurpali

Active fractions exhibiting anti-tuberculous activity from the plant(family&#x2014;Sterculiaceae) are described. Methanol extracts of the aerial part ofexhibited activity against MtbGS in primary screening of biosynthetic assay. Subsequent fractionation was carried out in organic solvents. Out of all these, two fractions (fraction D and K) are exhibiting inhibitory activity againstglutamine synthetase activity. These fractions D and K are inhibiting 74% and 44% respectively at 7.5 mg/ml concentrations. The determined ICvalue of the fraction K is found to be 4.5 mg/ml from the dose response curve against the purifiedGlutamine Synthetase. The ICvalue forBCG is 1.56 &#x3bc;g/ml. It was observed that there was no significant effect on proliferation of HL-60 cell line at 10&#xd7;MIC levels of the hits. This data indicated that these compounds could be initially considered to be safe.

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Maharashtra, India

Upasana Singh