Laboratoires Goemar S.a.

Centre National De La Recherche Scientifique

Laboratories Goemar S.a.

Tristan Barbeyron

Jean-Claude Yvin

Philippe Potin

Bernard Kloareg

Christophe Richard

Bernard Henrissat

William Helbert

Bernard Fritig

Jean-Marie Joubert

Bernard Cloarec

Mirjam Czjzek

Bertrand Plesse

Gurvan Michel

Valérie Descamps

Didier Flament

Olivier Klarszinsky

Jan-Hendrik Hehemann

Gaëlle Correc

Gaeelle Correc

The present invention relates to the isolation and to the characterization of two proteins having a novel enzymatic activity, i.e. a porphyranase activity. These proteins are useful for hydrolyzing polysaccharides containing sulfated agaro-colloids and for producing oligo-porphyrans, notably oligo-porphyrans with a defined structure and size.

Porphyranases, and use thereof for hydrolyzing polysaccharides

The invention concerns endofucanases suitable for preparing fuco-oligosaccharides by enzymatic hydrolysis of fucanes. It also concerns a bacterium producing endofucanases and the preparation of said enzyme from the bacterium. It further concerns endofucanase genes and it finally concerns the use said fuco-oligosaccharides for plant protection.

Endofucanases and method using same for preparing fuco-oligosaccharides from fucanes, bacterium producing endofucanases and uses of fuco-oligosaccharides for plant protection

The present invention relates to genes which code for glycosyl hydrolases having an HCA score with the iota-carrageenase of which is greater than or equal to 65% over the domain extending between amino acids 164 and 311 of the protein sequence SEQ ID No. 2 of said iota-carrageenase, and to genes which code for glycosyl hydrolases having an HCA score with the kappa-carrageenase of which is greater than or equal to 75% over the domain extending between amino acids 117 and 262 of the protein sequence SEQ ID No. 6 of said kappa-carrageenase.

Glycosyl hydrolase genes and their use for producing enzymes for the biodegradation of carrageenans

The invention relates to the novel strain deposited in the DSMZ Collection (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (German Collection of Microorganisms and Cell Cultures)) on May 8, 1998 under the number DSM 12170, the agaA gene coding for a &bgr;-agarase and having SEQ ID No. 1, the agaB gene coding for a &bgr;-agarase and having SEQ ID No. 3, said genes coding for a &bgr;-agarase of DSM 12170, and the specific nucleic acid sequence of the agaA gene coding for a specific peptide fragment AgaA&prime; which has conserved &bgr;-agarase activity, and having SEQ ID No. 5, as well as the protein AgaA of DSM 12170 having SEQ ID No. 2, the protein AgaB of DSM 12170 having SEQ ID No. 4 and said peptide fragment AgaA&prime; of DSM 12170 having SEQ ID No. 6.

Genes coding for &bgr;-agarases and their use for producing agar biodegradation enzymes

The present invention relates to genes which code for glycosyl hydrolases having an HCA score with the iota-carrageenase of,which is greater than or equal to 65% over the domain extending between amino acids 164 and 311 of the protein sequence SEQ ID No. 2 of said iota-carrageenase, and to genes which code for glycosyl hydrolases having an HCA score with the kappa-carrageenase of,which is greater than or equal to 75% over the domain extending between amino acids 117 and 262 of the protein sequence SEQ ID No. 6 of said kappa-carrageenase.

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Cleder, France

Tristan Barbeyron