Ajinomoto Co., Ltd.

Toshiki Taba

Shunichi Suzuki

Seiichi Hara

Yasuaki Takakura

Kenzo Yokozeki

Kunihiko Watanabe

Masakazu Sugiyama

It is an object of the present invention to provide a procedure for realizing inexpensive and simple production of 3-indole-pyruvic acid. A transformant is made using a polynucleotide having a specific nucleotide sequence encoding a protein having an oxidase activity, and oxidase is generated by culturing the transformant in a medium to accumulate the oxidase in the medium and/or the transformant. Further, tryptophan is converted into 3-indole-pyruvic acid in the presence of the transformant and/or a culture thereof to produce 3-indole-pyruvic acid.

Methods for making 3-indole-pyruvic acid from tryptophan using a tryptophan deaminase

Oxidase gene and method for producing 3-indole-pyruvic acid by utilizing the gene

The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and an aldolase which can be used in these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid).

Method for producing optically active IHOG and monatin

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Kanagawa, Japan

Toshiki Taba