US Government As Represented by the Secretary of the Army

The United States Government As Represented by the Department of Veterans Affairs

American Registry of Pathology

The United States of America As Represented by the Secretary of Defense on Behalf of the Armed Forces Institute of Pathology

Timothy J O'Leary

Jeffrey T Mason

Carol B Fowler

Robert E Cunningham

A method is disclosed for reversing fixation-induced cross-linking in tissue specimens that have been preserved for histological examination. The method involves placing the fixed tissue in a liquid under elevated temperature and pressure conditions that are sufficient to reverse the fixation-induced cross-linking, restore antigenicity to proteins, and permit improved molecular and proteomic analysis of the preserved tissue specimen. Methods are also disclosed for processing tissues for histological examination under elevated pressure conditions that enhance the perfusion of liquid reagents into the tissue and reduce overall processing times.

Pressure-assisted molecular recovery (PAMR) of biomolecules, pressure-assisted antigen retrieval (PAAR), and pressure-assisted tissue histology (PATH)

Immunoliposomes and use thereof in highly specific and sensitive nucleic acid amplification assays relying on amplification of specific nucleic acid sequences released from encapsulation within a liposome after a receptor on the liposome couples with a targeted analyte/antigen immobilized on a select surface. The immunoliposome nucleic acid amplification assay permits both quantitative and qualitative analyte detection.

Immunoliposome-nucleic acid amplification (ILNAA) assay

Growing community of inventors

Washington, DC, United States of America

Timothy J O'Leary