University of Missouri

The Penn State Research Foundation

Wisconsin Alumni Research Foundation

Other

Leland Stanford Junior University

Timothy E Glass

Kenneth Hettie

Ronald Thaddeus Raines

Luke D Lavis

Kevin Gillis

Sunil S Chandran

Jessica L Klockow

Frederick T Chin

Joseph Raker

Ricardo Moran

Kristen Secor

The present disclosure encompasses embodiments of a novel near-infrared-emitting molecular fluorophore and probes incorporating said fluorophore advantageous for in vitro and in vivo research studies. The fluorophore is robust, photostable, and possesses functionalities for easy bioorthogonal conjugation (e.g., click chemistry, hydrazone formation, Diels Alder, Staudinger ligation, etc.). It is biocompatible and emits at 711 nm in aqueous conditions. These fluorophores may be used to fluorescently tag biological molecules or structures of interest, or used as optical reporters (i.e., activatable molecular probes, fluorescent dyes) for specific biomarkers/analytes as they can be switched from 'off' to 'on.' This fluorophore is useful for cellular assays and preclinical small animal imaging as the near-infrared emission is highly penetrating, and the photophysical properties are outstanding. As such, the properties of this class of fluorophores could easily be translated for use in clinical applications.

Rhodol fluorophores for near-infrared imaging

The invention is provides for a variety of fluorescence sensing compounds characterized generally as having a coumarin-3-aldehyde scaffold with a pendant aryl moiety at the C4-position of the scaffold. The further provides a method for detecting primary amines in a biological sample using said compounds. Especially, for the selective labeling and imaging of catecholamines in live and fixed secretory cells.

Fluorescent chemical sensor for biological amines

A fluorescence sensing compound for separately detecting and visualizing one or more monoamine neurotransmitters in cells, the fluorescence sensing compound having the following formula: 

Near-infrared fluorescent sensors for biological amines

The invention is directed to dual-analyte fluorescent chemosensors for the direct detection and visualization (imaging) of neurotransmitters released upon exocytosis. The inventive sensor exploits the high concentration of neurotransmitters (e.g., glutamate, norepinephrine, and dopamine) and capitalizes upon the pH gradient between the vesicle and synaptic cleft.

pH-sensitive fluorescent sensors for biological amines

Latent fluorescent compounds, comprising a fluorescent molecule with one or more blocking groups attached and optionally one or more urea-containing groups are provided. The urea-containing group can be used to further attach one or more molecules of interest, such as proteins, peptides or nucleic acids. The blocking group(s) is released from the latent fluorescent compound by reaction with a trigger, forming the fluorescent molecule which can be detected. Also provided herein are methods of using latent fluorescent compounds to detect triggers.

Fluorescence assays with improved sensitivity

The invention provides compounds of formulas I, II, and III, methods of making them, and methods of their use. The compounds of the invention can be used as fluorescent sensors, for example, to detect an amine-containing analyte in a biological sample. The compounds can be selective for one type of amine over others and the amount of fluorescence can be correlated with the concentration of the amine in the sample.

Fluorescent sensors for cellular amines

An article storage display tower for storage, display and continuous easy access to the article is provided. The display tower has a top and a bottom with a cell structure having a plurality of cells positioned between the top and the bottom. Each of the cells of the tower can store and display a plurality of articles, such as compact discs, while allowing access to the articles. The cell structure is rotatably connected to a base that permits the cell structure to rotate about an axis perpendicular to the base, so that access to all cells is facilitated.

Article storage display tower

Chemical sensor compositions and methods for quantifying analytes are described. The chemical sensors include analyte-binding moieties and reporter moieties covalently attached to a framework including two trityl groups connected by a linear spacer such as ethyne or butadiyne. The sensors chelate an analyte across the acetylene axis of the molecule to stabilize the molecule in an eclipsed rotamer conformation. This conformation causes the sensors to emit a measurable signal. Also disclosed are methods of making the chemical sensors, methods of using the chemical sensors, and kits for quantifying analytes using the chemical

Growing community of inventors

State College, PA, United States of America

Timothy E Glass