Other

Jiangsu Micromedmark Biotech Co., Ltd.

Beijing Dcty Biotech Co., Ltd.

Tianfu Zhang

Chenyu Zhang

Ke Zeng

Rong Zhang

Gang Peng

Hongwei Gu

Shunchang Jiao

Zishan Zhou

Jiasen Xie

Ziming Wu

Some embodiments of the disclosure use human peripheral blood for ctDNA sequencing or tumor tissues for whole exome sequencing, screen out mutation sites to perform antigen epitope prediction, connect and synthesize an expression gene sequence of mutant peptides. Other embodiments of the disclosure construct a lentiviral vector, package the lentivirus, transfect an APC cell to complete transformation of a specific LV cell, co-culture in vitro with PBMC separated from the peripheral blood, screen out an effective polypeptide, and transform common T cells into RFF cells. Suppressive signaling molecules include one or more of PD-1, Tim-3, LAG3, CTLA-4, BTLA, VISTA, CD160, and 2B4 (CD244). Antigen presenting cells include one or more of PBMC, a dendritic cell, neutrophil, B lymphocyte, and macrophage.

LRFFT2 cell

The present invention providesnon-coding RNA and the identification and application thereof. In particular, the present invention proves through experiments that abacterium delivers non-coding RNAs (ncRNAs) encoded by the bacterium itself into a host cell, generates milRNAs similar to microRNAs by means of a microRNA splicing system in the cell, regulates the immune system with the milRNAs, and further protects thebacterium from being cleared by the host. Adsorbing milRNAs using milRNA inhibitors can effectively inhibit the survival ability of the bacterium in the cell, leading to a reduced vitality of the bacterium. The present invention further provides relevant reagents and methods for effective detection of, treatment to and study onbacteria orinfectious diseases.

[object Object]

Growing community of inventors

Beijing, China

Tianfu Zhang