Novozymes A/s

Thomas Sch&auml;fer

Allan Svendsen

Carsten Schau Andersen

Joel R Cherry

Lars Beier

Lene Lange

Torben Peter Frandsen

Thomas Sandal

Carsten Sj&oslash;holm

Fiona Duffner

Jack Beck Nielsen

The present invention relates to a method of generating a gene library from an environmental pool of organisms, which gene library is enriched in DNA encoding a polypeptide with an activity of interest. Also, the invention provides a method of selecting a DNA sequence of interest from an environmental pool of organisms. Further, the invention relates to a gene library prepared from an enriched environmental pool of organisms enriched in DNA encoding a polypeptide with an activity of interest.

Method for generating a gene library

The inventors have discovered some striking, and not previously predicted structural similarities and differences between the structure of Novamyl and the reported structures of CGTases, and based on this they have constructed variants of maltogenic alpha-amylase having CGTase activity and variants of CGTase having maltogenic alpha-amylase activity. Further, on the basis of sequence homology between Novamyl&reg; and CGTases, the inventors have constructed hybrid enzymes with one or more improvements to specific properties of the parent enzymes, using recombinant DNA methodology.

Amylolytic enzyme variants

A process for preparing a dough or a baked product comprises adding an amylase to the dough in an amount which is effective to retard the staling of the bread. The amylase is an exo-amylase which hydrolyzes starch to form mainly mal-totriose.

Preparation of dough and baked products

Growing community of inventors

Farum, Denmark

Thomas Schäfer

Growing community of inventors

Farum, Denmark

Thomas Sch&auml;fer

Thomas Schäfer