Harvard College

Massachusetts Institute of Technology

Other

Whitehead Institute for Biomedical Research

Whitehead Insti.

Thomas P Maniatis

Phillip A Sharp

Michael J Lenardo

Albert S Baldwin

David Baltimore

Louis Staudt

Harinder Singh

Ranjan Sen

Lynn M Corcoran

Roger G Clerc

Patrick A Baeuerle

Jonathan H Lebowitz

Chen-Ming Fan

Vito J Palombella

Alfred L Goldberg

Richard M Myers

Oliver Rando

Disclosed herein is a method for regulating the activity of NF-&kgr;B in an animal comprising contacting cells of the animal with certain proteasome inhibitors.

Proteasome regulation of NF-KB activity

Constitutive and tissue-specific protein factors which bind to transcriptional regulatory elements of Ig genes (promoter and enhancer) are described. The factors were identified and isolated by an improved assay for protein-DNA binding. Genes encoding factors which positively regulate transcription can be isolated and employed to enhance transription of Ig genes. In particular, NF-kB, the gene encoding NF-kB, IkB and the gene encoding IkB and uses therefor.

Nuclear factors associated with transcriptional regulation

Nuclear factors associates with transcriptional regulation

A method for detecting and localizing single base substitutions in RNA or DNA that involves RNAase A cleavage of single base mismatches in RNA:RNA or RNA:DNA heteroduplexes. A RNA probe complementary to wild type DNA is annealed to the test DNA containing a single base substitution. Many of the possible single base mismatches can be cleaved by RNAase A. The location of the single base substitution can be determined by analyzing the sizes of the RNA cleavage products.

Detection of base pair mismatches using RNAase A

Growing community of inventors

Belmont, MA, United States of America

Thomas P Maniatis