Nutrinova Nutrition Specialties & Food Ingredients Gmbh

Nutrinova Inc.

Nutrinova Nutrition Specialities & Food Ingredients Gmbh

Other

Hoechst Aktiengesellschaft

Aventis Research & Technologies Gmbh & Co Kg

Axiva Gmbh

Hoechst Research & Technologies Gmbh & Co. Kg

Nutrinova, Nutritition Specialties & Food

Nutrinova Nutrition Specialties & Food Engredients Gmbh

Adventis Research & Technologies Gmbh & Co. Kg

Thomas Kiy

Dirk Fabritius

Matthias Rüsing

Lothar Elling

Maria Regina Kula

Petra Dresler

Joachim Schultz

Wolfgang Stock

Thomas Schweins

Alexander Leske

Tanja König

Anton Van V Putten

Stefan Mullner

Dieter Wullbrandt

Markus Luy

Annette Dominitzki

Matthias Rusing

Joern Plote

Joem Plote

Matthias R&uuml;sing

R&uuml;diger Marquardt

Heinz Engelhardt

Christoph Siffrin

Ulrich Klein

Gunther Steinbruck

Anton Van Putten

The invention relates to genes which are coded for sequences specific to polyketide synthases (PKS). The thus synthesized PKS is characterized by the enzymatic capacity thereof to produce PUFAs (polyunsaturated fatty acids). The invention also relates to the identification of the corresponding DNA-sequences, in addition to the use of said nucleotide sequences for the production of recombined and/or transgenic organisms.

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The invention relates to a method for the fermentative production of lipids/fatty acids having high concentrations of omega-6 fatty acids (PUFA, polyunsaturated fatty acids), especially &#x3b3;-linolenic acids (GLA) in ciliates. Said lipids/fatty acids can be used in human/animal food, and in the pharmaceutical field.

Method for producing &#x3b3;-linolenic acids from a ciliate culture by adding suitable precursor molecules to said culture medium

The present invention relates to nucleotide-sugar-synthesizing enzymes (enzymes with nucleotidyltransferase or pyrophosphorylase activity) from nonparasitic protists, to a process for the preparation thereof and to the use thereof for preparing nucleotide-sugars. The enzymes according to the invention make possible or greatly simplify the enzymatic preparation of various nucleotide-sugars on the industrial scale from low-cost precursors. It is possible with the aid of the discovered enzymes to prepare, for example, GDP-fucose, GDP-mannose, UDP-glucose, UDP-glucosamine, UDP-galactose, UDP-galactosamine, UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine in economic quantities.

Nucleotide-sugar-synthesizing enzymes from nonparasitic protists

The invention relates to nucleic acid(s) which is/are obtained from tetrahymena and which code(s) for a ciliate-specific delta-6-desaturase that is involved in the biosynthesis of commercially valuable, multiply unsaturated fatty acids (so-called PUFA) polyunsaturated fatty acids). The inventive nucleotide sequence and the polypeptide sequence that can be obtained therefrom exhibit a surprisingly low sequence identity compared to other known natural desaturases. The invention also relates to the use of the nucleic acid(s) for overexpression in ciliates, preferably tetrahymena, in particular,with the aim of increasing the production of delta-6 unsaturated fatty acids, especially GLA.

The present invention relates to nucleic acids isolated from Tetrahymena which code for a ciliate-specific triterpenoid cyclase. The inventive nucleotide sequences and the polypeptide sequences derived therefrom demonstrate a surprisingly minimal sequence identity to known isoprenoid cyclases. The invention also relates to the use of nucleic acids for the regulation of triterpenoid cyclase expression in a host organism, as well as the targeted knockout or repriming of the triterpenoid cyclase gene. As a result of the altered expression of the triterpenoid cyclase, it is possible to modify and enrich the levels of multiple unsaturated fatty acids in the host organism.

The intention relates to a method for the fermentative production of lipids/fatty acids having high concentrations of omega-6 fatty acids (PUFA, polyunsaturated fatty acids), especially &#x3b3;-linolenic acids (GLA) in ciliates. Said lipids/fatty acids can be used in human/animal food, and in the pharmaceutical field.

The present invention concerns a method for production of recombinant human proteins, in whichcells are transformed with recombinant DNA containing at least one functional gene that codes for a human protein, the recombinantcells are cultured, in which the gene that codes for a human protein is expressed and the proteins are then isolated. The present invention also concerns a corresponding method, in which the gene that codes for a human protein contains a human leader sequence that leads to secretion of the expressed protein.

Expression of recombinant human proteins in Tetrahymena

The invention relates to the isolation of the prepro form of cathepsin L, of its leader sequence, of cathepsin L and of the affiliated propeptide from ciliates, in particular Paramecium, to the use of these peptides and to a process for preparing cathepsin L from ciliates.

Cathepsin L, its prepro form and the corresponding propeptide from ciliates

In the fermentation process according to the invention with continuous mass cultivation of ciliates (protozoa), the ciliate cells are cultivated in a complex axenic medium&mdash;free from living feed or prey organisms&mdash;and the biomass containing the desired biogenous valuable substances is obtained by continuous (permanent) cell extraction.

Fermentation method with continuous mass cultivation of ciliates (protozoa) for producing biogenous valuable substances

Nucleic acid isolated from Tetrahymena which codes for a triterpenoid cyclase, its production, and use

The invention relates to a method for culturing ciliates comprising the steps of placing ciliates and medium therefor in a culture flask; providing said culture flask with a stirrer having a magnetic core, wherein said stirrer is suspended in the top part of the culture flask such that the stirrer does not touch the flask bottom, and wherein the motion of said stirrer is driven by means of a magnetic field; and stirring said ciliates in said culture medium.

Method for culturing ciliates

The present invention relates to a process for the preparation of &ggr;-linolenic acid (GLA) from Colpidium and its use in foodstuffs, cosmetics and pharmaceuticals. Colpidium can be fermentatively cultured up to a high absolute yield of GLA in an optimized medium.

Obtainment of &ggr;-linolenic acid from protozoa of the genus colpidium

The invention relates to a method for obtaining fatty acid esters and fatty acids, preferably unsaturated fatty acid ethyl esters, from biological sources by continuous, combined in-situ extraction, reaction and chromatography. The invention is characterized in that simultaneously and in the same location, in the presence of a compressed gas and an 0.5-5 % stream of lower alcohols as modifiers and in the presence of an inert catalyst, the fatty acids are fully transesterified from their native fatty acid sources, which are selectively desorbed and eluated in the conditions specified above.

Method for obtaining fatty acids from biomass by combined in/situ extraction, reaction and chromatography using compressed gases

Cathepsin-L, the pre-proform thereof and the corresponding propeptide from ciliates

UDP-glucose pyro phosphorylase enzymes from nonparasitic protozoa

Growing community of inventors

Frankfurt-am-Main, Germany

Thomas Kiy