University of Texas System

Thomas G Wood

Allan R Brasier

Pomila Singh

Yingxin Zhao

Steven G Widen

Tao Hai

Yuanfen Wei

DNA aptamers are high affinity ligands selected by genetic enrichment techniques to bind to specific protein targets. Because these represent chemically stable and reproducible molecules, they have application as affinity reagents and/or therapeutic drugs to affect the target protein's actions. NF-kB is an important mediator of the innate immune response and mediator of tissue inflammation. Although RNA and double stranded DNA aptamers have been identified to bind to the NF-kB family of proteins, the present invention represents the first identification of single stranded DNA aptamers that recognize NFkB RelA. The aptamers disclosed herein bind to several distinct regions of RelA and may be useful to antagonize the DNA binding of RelA as an inhibitor of cellular inflammation, visualize the location or amount of RelA in tissues from pathological conditions, or to quantitatively measure the activated state of RelA by affinity binding.

Single stranded DNA aptamers binding NF-kB/RelA

Single stranded DNA aptamers binding NF-&#x3ba;B/RelA

Disclosed are isolated IKK&#x3b3;&#x394;; isolated nucleic acid molecules encoding IKK&#x3b3;&#x394;; expression vectors and cells which contain such nucleic acid molecules; oligonucleotides which are complementary to at least a portion of nucleic acid molecules encoding IKK&#x3b3;&#x394; but which are not complementary to any portion of nucleic acid molecules encoding wild type IKK&#x3b3;; antibodies which are specific for IKK&#x3b3;&#x394;; DNA and RNA oligomers, optionally in labeled or enzymatically active form, which are capable of selectively hybridizing to nucleic acid molecules encoding IKK&#x3b3;&#x394;; and DNA and RNA oligomers, optionally in labeled or enzymatically active form, which are capable of selectively hybridizing to nucleic acid molecules encoding IKK&#x3b3;&#x394; but which do not hybridize with nucleic acid molecules encoding wild type IKK&#x3b3;. Methods for using these materials to inhibit formation of IKK&#x3b3;&#x394;-containing IKK, to inhibit NF-&#x3ba;B activation, and to treat or prevent inflammation are also disclosed.

IKK gamma gene products and methods for making and using same

The present invention discloses methods for the treatment of colon cancer. The expression of gastrin by colon cancers is inhibited by the use of antisense gastrin expression. Methods are disclosed for the preparation of expression constructs and the use of such constructs to inhibit colon cancer growth.

Antisense polynucleotide

The present invention discloses is for the treatment of colon cancer. The expression of gastrin by colon cancers is inhibited by the use of antisense gastrin expression. Methods are disclosed for the preparation of expression constructs and the use of such constructs to inhibit colon cancer growth.

Growing community of inventors

Houston, TX, United States of America

Thomas G Wood