Dyax Corporation

Other

Thomas Cushman Ransohoff

Robert Charles Ladner

John Moore Maclennan

Peter J Leavesley

Jeffrey R Green

Jeffrey A Kaster

Peter C Van Davelaar

Christine M Conroy

Gary L McNeil

Victor R Lancaster

A method is disclosed for obtaining affinity ligands for isolating tissue-type plasminogen activator (tPA). Ligands binding tPA with high specificity at pH 7 and releasing tPA at pH 5 or lower are disclosed. Also disclosed are methods whereby additional ligands having desirable preselected binding and release (elution) characteristics may be isolated, permitting the development of tailored ligands to meet the purification problems presented by any particular feed stream containing tPA.

Purification of tissue plasminogen activator (tPA)

Purification of tissue plasminogen activator (TPA)

A chromatography cartridge assembly includes a cartridge and first and second end caps. A wall of the cartridge defines a chamber for containing chromatography media. The end caps define inlet and outlet passages, respectively, for flow of process fluid. The first end cap defines flow distributor passages for distributing process fluid across a cross-sectional area of the chamber, and the second end cap defines flow collector passages for collecting process fluid from across a cross-sectional area of the chamber. A clamp is circumferentially located about the cartridge wall for applying a radial load through the wall to the first end cap to fix the first end cap in a desired position.

Liquid chromatography column

A method is disclosed for obtaining affinity ligands for isolating tissue-type plasminogen activator (tPA). Ligands binding tPA with high specificity at pH 7 and releasing tPA at pH 5 or lower are disclosed. Also disclosed are methods whereby additional ligands having desirable preselected binding and release (elution) characteristics may be isolated, permitting the development of tailored ligands to meet the purification problems presented by any particular feed stream

Polypeptides that bind to tissue plasminogen activator (tPA)

A chromatography cartridge assembly includes a cartridge with a flexible wall defining a chamber, and a porous, polymeric, hydrophilic, chromatography media contained within the chamber. The chromatography media has an operating pressure rating greater than 3 bars. The flexible wall forms a movable diaphragm for compressing the chromatography media. The chromatography cartridge assembly includes a flow distributor, flow collector, and sieves formed from a hydrophilic material. A compression module defines a pressure chamber for containing a pressurized fluid. The pressurized fluid acts to move the flexible wall of the cartridge assembly. The compression module has a pressure ratings greater than 3 bars.

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Lexington, MA, United States of America

Thomas Cushman Ransohoff