Hoffmann-la Roche Inc.

Carl Zeiss Jena Gmbh

F. Hoffmann-la Roche Ag

Cybio Ag

Thilo Enderle

Doris Roth

Eric Argirios Kitas

Christof Fattinger

Cornelia Hertel

Martin Graf

Sannah Jensen Zoffmann

Hugues Matile

Hansj&ouml;rg Tschirky

Martin Gluch

Hansjoerg Tschirky

Richard Ameling

Gerhard Flury

Hans-Peter Hirt

The present invention relates to an in vitro method for detecting cAMP or cGMP comprising a) contacting a mixture with a complex of a tracer and a dequencher, wherein the tracer is a fluorophore covalently linked to a cAMP quencher, and b) measuring the change in fluorescence. Furthermore, the present invention relates to the use of said method for determining the cAMP or cGMP concentration in the mixture, for determining the activity of a receptor wherein the signal transduction of the receptor comprises cAMP or cGMP and for screening of a ligand for such a receptor.

Method of detection of cAMP and cGMP via a fluorophore and dequencher complex

The present invention relates to a generic method for detecting a kinase or phosphatase activity. The method comprises the following steps: incubating a kinase or phosphatase activity sample with a kinase or phosphatase substrate molecule comprising either a fluorophore having a detectable readout or molecule with an aromatic group which serves as a quencher of the fluorophore, incubating the mixture of step a) with a detection entity comprising either a fluorophore or a molecule with an aromatic group and a binding partner, wherein the substrate molecule and the detection entity are capable of binding to the binding partner and the binding of the substrate molecule and the detection entity to the binding partner lead to an altered readout of the fluorophore, and measuring the readout of the fluorophore in the mixture of step b), wherein an altered readout of the fluorophore compared to a blank is indicative for the presence of a kinase or phosphatase activity in the sample.

Generic kinase/phosphatase assay with single readout

The present invention relates to a method of determining kinase or phosphatase activity based on a three parts system. The method comprises contacting a binding partner which can bind phosphorylated peptides, a detection molecule and a substrate peptide. Determination of activities is achieved by measuring energy transfer between an energy donor and an energy acceptor that are present on the detection molecule and the substrate molecule.

Three part assay for kinase or phosphatase activity

A transport system for transporting and handling microtiter plates, for use in high throughput screening, diagnosis and/or combinatorial chemistry, comprising modules with devices for preparing specimens and/or introducing specimens, and/or for optical readout and/or for plate storage and/or devices for further processing steps or readout steps. The system includes an inter-modular transport system for transporting the microtiter plates between the different devices and at least one central transport system for asynchronous plate transfer between individual modules via input and output buffers.

Device for transporting and holding microtiter plates

The present invention relates to a fluorescently labeled growth hormone secretagogue which can be used for the identification of compounds capable of binding to growth hormone secretagogue receptors, in particular by high throughput screening.

Fluorescently labeled growth hormone secretagogue

A microdispensing system is useful for the open-jet dispensing of liquids. A valve having a valve chamber is bounded by an impermeable elastic membrane and is filled with the liquid for dispensing. One or more liquid supply lines lead to the valve chamber. An outlet closed from the exterior by the membrane in the position of rest leads from the chamber to a dispensing nozzle. An actuator chamber is disposed on the other side of the membrane. In the actuator chamber, an electromechanical drive is connected to the membrane for the purpose of briefly lifting the membrane from the outlet. The actuator chamber is closed in such manner that it can be subjected to a pressure differing from atmospheric pressure.

Microdispensing system for the open-jet dispensing of liquids

Growing community of inventors

Rheinfelden, Germany

Thilo Enderle