Ajinomoto Co., Ltd.

Tetsuo Tanegawa

Kenji Fujiwara

Tetsuya Kawakita

Masaru Saeki

Shigeyoshi Miyashiro

Kiyohiko Nishi

Takeru Sato

Seiji Toyoshi

Hirofumi Tasaki

Masayoshi Kusatsu

Masaki Koga

Hiroshi Kaneko

Seisaku Furuya

The present invention provides a method of manufacturing a highly pure ammonium succinate solution including the steps of (A) producing calcium succinate trihydrate by crystallization fermentation of a microorganism; (B) converting calcium succinate trihydrate to calcium succinate monohydrate by transition crystallization; (C) separating the calcium succinate monohydrate crystals; (D) substituting the calcium salt in the calcium succinate monohydrate with ammonium salt resulting in an ammonium succinate solution; and (E) removing the solid calcium carbonate from the ammonium succinate solution.

Method of manufacturing succinic acid and ammonium succinate solutions

The present invention relates to a water-insoluble glucan purification method characterized in that a culture solution of a microbe that produces water-insoluble glucan or a microbial cell-containing solution comprising water-insoluble glucan as a component is treated with both an oxide of 0.175-3.5 wt.--wt. % and a hydroxide such that the pH value becomes 10-12.5. Since the present invention accomplishes all of the destruction of production microbe, the decolorization and the reduction of viscosity of a culture solution of a microbe that produces water-insoluble glucan or a microbe cell-containing solution comprising water-insoluble glucan as a component, the invention purifies water-insoluble glucan industrially at low costs and with a high efficiency.

Water-insoluble glucan purification method

A method for removing impurities including humic substances, gums, polysaccharides, proteins or a mixture thereof from an amino acid fermented liquor obtained from cane, beet molasses, or a mixture thereof, said amino acid being selected from the group consisting of glutamic acid, lysine, and a mixture thereof; said method comprising:

Ultrafiltration method

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Saga, Japan

Tetsuo Tanegawa