Industrial Technology Research Institute

National Health Research Institutes

Obi Pharma Inc.

Teng-Yi Huang

Cheng-Der Tony Yu

Hsiao-Wen Chung

Wen-Yih Isaac Tseng

Tai-Xing Yu

Ting-Kai Hung

Yi-Chien Tsai

Nan-Hsuan Wang

Yin-Cheng Hsieh

Hsu-Hsia Peng

Hong-Chi Hsu

Li-Chung Chuang

Yih Huang Hsieh

Pu-Ling Hu

The present disclosure relates to novel glycosynthase enzymes for glycoprotein engineering and/or homogeneous antibody remodeling. The enzyme variants, termed EndoSd-D232M and EndoSz-D234M, contain the glycan conjugation and/or modification activity at the conserved N297 glycosylation site of Fc region of an exemplary antibody. It has been demonstrated that the glycosynthase activities of EndoSd-D232M and EndoSz-D234M can be applied to various mAbs targeting different receptors, including, but not limited to, Globo H, SSEA-4, SSEA-3 series of receptors (OBI-888; Globo H ganglioside), Herceptin (Her 2 receptor), Perj eta (Her 2 receptor) and Vectibix (EGFR receptor). It has been found that both mAb-GlcNAc and mAb-GlucNAc(F) were suitable substrates for both EndoSd-D232M and EndoSz-D234M. The ADCC assay of related products, OBI-888-G2S2 and Herceptin-G2S2, showed that the remodeled homogeneous antibody, mAb-G2S2, has an increased relative activity ranging from 3 to 26 folds.

Glycosynthase variants for glycoprotein engineering and methods of use

In one aspect, in general, a method is provided for detecting temperature and protein denaturation of a tissue during thermal therapy. The method includes generating a plurality of MR pulse sequences that include a first group of pulse sequences and a second group of pulse sequences, and receiving a plurality of response signals that include a first and second group of response signals in response to the first and second groups of pulse sequences, respectively. A first information associated with a degree of protein denaturation of the tissue is determined based on the first and second groups of response signals. A second information associated with a temperature of the tissue is determined based on at least some of the plurality of response signals.

Detecting temperature and protein denaturation during thermal therapy

A compressor/decompressor (Codec) selecting apparatus includes a performance analyzer, a training server, a storage unit, and a selecting unit. The performance analyzer analyzes the performance of the current internet communication system, and outputs environmental parameters accordingly. The training server obtains learning data corresponding to a hyperplane according to training samples. The storage unit stores the learning data and the related parameters. The selecting unit does a functional analysis according to the environmental parameters and the learning data in order to select the Codec suitable for the current internet communication system.

Compressor/decompressor selecting apparatus and method of the same

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Taipei, Taiwan

Teng-Yi Huang