Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo

Takanobu Higashiyama

Michio Kubota

Toshio Miyake

Shigeharu Fukuda

Keiji Tsusaki

Hikaru Masahiro Watanabe

Tomoyuki Nishimoto

Hajime Aga

Tomohiko Sonoda

The object of the present invention is to provide an &#x3b1;-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an &#x3b1;-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the &#x3b1;-1,6 glucosidic linkage as a linkage at the non-reducing end and the &#x3b1;-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the &#x3b1;-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the &#x3b1;-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; &#x3b1;-isomaltosyl-transferring method using the enzyme; method for forming &#x3b1;-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-&#x3b1;-D-glucopyranosyl-(163)-&#x3b1;-D-glucopyranosyl-(166)-&#x3b1;-D-glucopyranosyl-(163)-&#x3b1;-D-glucopyranosyl-(16} using both the &#x3b1;-isomaltosylglucosaccharide-forming enzyme and the &#x3b1;-isomaltosyl-transferring enzyme; and the uses of the saccharides obtainable therewith.

&#x3b1;-isomaltosylglucosaccharide-forming enzyme, process and uses of the same

A novel process for producing isomaltose and uses thereof and comprising the steps of contacting saccharides, which have a glucose polymerization degree of at least two and &#x3b1;-1,4 glucosidic linkage as a linkage at the non-reducing end, with an &#x3b1;-isomaltosylglucosaccharide-forming enzyme, in the presence or the absence of &#x3b1;-isomaltosyl-transferring enzyme to form a-isomaltosylglucosaccharides, which have a glucose polymerization degree of at least three, &#x3b1;-1,6 glucosidic linkage as a linkage at the non-reducing end, and &#x3b1;-1,4 glucosidic linkage as a linkage other than the non-reducing end, and/or to form cyclo{&#x2192;6)-&#x3b1;-D-glucopyranosyl-(1&#x2192;3)-&#x3b1;-D-glucopyranosyl-(1&#x2192;6)-&#x3b1;-D-glucopyranosyl-(1&#x2192;3)-&#x3b1;-D-glucopyranosyl-(1&#x2192;}; contacting the saccharides so formed with isomaltose-releasing enzyme to release isomaltose; and collecting the released isomaltose; and uses thereof.

Process for producing isomaltose and uses thereof

The object of the present invention is to provide an &#x3b1;-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an &#x3b1;-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the &#x3b1;-1,6 glucosidic linkage as a linkage at the non-reducing end and the &#x3b1;-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the &#x3b1;-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the &#x3b1;-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; &#x3b1;-isomaltosyl-transferring method using the enzyme; method for forming &#x3b1;-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{&#x2192;6)-&#x3b1;-D-glucopyranosyl-(1&#x2192;3)-&#x3b1;-D-glucopyranosyl-(1&#x2192;6)-&#x3b1;-D-glucopyranosyl-(1&#x2192;3)-&#x3b1;-D-glucopyranosyl-(1&#x2192;} using both the &#x3b1;-isomaltosylglucosaccharide-forming enzyme and the &#x3b1;-isomaltosyl-transferring enzyme; and the uses of the saccharides obtainable therewith.

&#x3b1;-Isomaltosylglucosaccharide synthase, process for producing the same and use thereof

The object of the present invention is to provide a novel glycosyl derivative of cyclotetrasaccharide represented by cyclo{&#x2192;6)-&#x3b1;-D-glucopyranosyl-(1&#x2192;3)-&#x3b1;-D-glucopyranosyl-(1&#x2192;6)-&#x3b1;-D-glucopyranosyl-(1&#x2192;3)-&#x3b1;-D-glucopyranosyl-(1&#x2192;}, and it is solved by providing a branched cyclotetrasaccharide, wherein one or more hydrogen atoms in the hydroxyl groups of cyclotetrasaccharide are replaced with an optionally substituted glycosyl group, with the proviso that, when only one hydrogen atom in the C-6 hydroxyl group among the above hydrogen atoms is substituted with an optionally-substituted glycosyl group, the substituted glycosyl group is one selected from those excluding D-glucosyl group.

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Okayama, Japan

Takanobu Higashiyama