Mitsubishi Chemical Industries Limited

Seikagaku Kogyo Co. Ltd.

Tadashi Hishida

Akiyoshi Matsumoto

Akira Kamata

Yoshimasa Fujita

Isao Kawakami

Nobuo Imai

Isao Miyachi

Hiroshi Takamisawa

Isao Nakazawa

Tetsuya Hirota

Tsutomu Okada

Hachiro Ishikawa

Hideo Kato

Yusuke Maeda

Minoru Muramatsu

There is disclosed a method for assaying fatty acids in a system containing albumin together with the fatty acids using acyl-CoA synthetase, characterized by performing the assay in the presence of a water-soluble salt of a dibasic fatty acid having 10 to 18 carbon atoms or a benzenesulfonate optionally having one or more C.sub.1 -C.sub.5 side chains.

Method for assaying fatty acids

There is disclosed a process for the renewal of a deactivated insolubilized glucose isomerase which comprises subjecting an insolubilized glucose isomerase adsorbed onto an anion exchange resin, which has been used for isomerization of glucose into fructose to decrease its activity, to treatments by an aqueous mineral acid solution in combination with an aqueous alkaline solution, an aqueous electrolytic salt solution, an aqueous mineral acid or a mixture thereof to release adsorbed materials from the resin which is converted into a salt type and adsorbing fresh glucose isomerase to the regenerated resin to recover the activity of the insolubilized glucose isomerase.

Process for the renewal of an insolubilized glucose isomerase

A method for preservation of an aqueous glucose isomerase solution is disclosed in which an aqueous glucose isomerase solution is mixed with a an alkaline material to adjust its pH value within a range of from 9 to 12 whereby the aqueous solution can be stored at room temperature for a period not less than three days without microbial contamination, putrefaction, denaturation and deactivation of the glucose isomerase.

Method for preservation of aqueous glucose isomerase solution

There is disclosed a process for producing an insolubilized glucose isomerase useful for converting glucose into fructose comprising contacting a macroporous anion exchange resin with glucose isomerase to effect absorption of the isomerase on the resin, said resin having high porosity and high ion exchange capacity, whereby the resulted insolubilized glucose isomerase has high degree of adsorption, high activity retention and high activity yield.

Insolubilized glucose isomerase

There is disclosed a process for isomerization of glucose into fructose which comprises contacting an aqueous glucose solution with glucose isomerase in the presence of iron ion and magnesium ion, said glucose isomerase being in the form of insolubilized glucose isomerase adsorbed on a carrier.

Process for the isomerization of glucose into fructose

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Tokyo, Japan

Tadashi Hishida