Arcadia Biosciences, Inc.

Susan R Hurst

Dayna L Loeffler

Ann Joan Slade

Trenton G Colbert

Vic C Knauf

Michael N Steine

Claire McCallum

Anna Amen

Dionne Vafeados

Claire M Mccallum

A series of independent human-induced non-transgenic mutations found in an expansin gene (LeExp1) of tomato; tomato plants having these mutations in their LeExp1 genes; and a method of creating and identifying similar and/or additional mutations in the LeExp1 gene by screening pooled and/or individual tomato plants. The tomato plants of the present invention exhibit fruit that soften more slowly post-harvest without having the inclusion of foreign nucleic acids in their genomes.

Tomatoes that soften more slowly post-harvest due to non-transgenic alterations in an expansin gene

A series of independent human-induced, non-transgenic mutations found in at least one non-ripening (NOR) gene of tomato; tomato plants having these mutations in at least one of their NOR genes; and a method of creating and identifying similar and/or additional mutations in the NOR gene by screening pooled and/or individual tomato plants. The tomato plants of the present invention exhibit fruit that ripen more slowly, rot more slowly, are firmer, and have a longer shelf life post-harvest as a result of non-transgenic mutations in at least one of their NOR genes.

Non-transgenic tomato varieties having increased shelf life post-harvest

The present invention provides a series of independent human-induced, non-transgenic mutations found in at least one tomato &#x3b2;-galactosidase 4 gene, tomato plants having these mutations in at least one of their tomato &#x3b2;-galactosidase 4 genes, and a method of creating and identifying similar and/or additional mutations in the tomato &#x3b2;-galactosidase 4 gene by screening pooled and/or individual tomato plants. Tomato plants identified and produced in accordance with the present invention have fruit that are firmer when ripe with reduced post-harvest softening compared to fruit from wild type tomato plants as a result of non-transgenic mutations in at least one of their tomato &#x3b2;-galactosidase 4 genes.

Non-transgenic tomato varieties having increased shelf life post-harvest due to alterations in &#x3b2;-galactosidase 4

A series of independent non-transgenic mutations found in the fruit PG gene of tomato; tomato plants having these mutations in their fruit PG gene; and a method of creating and identifying similar and/or additional mutations in the PG gene by screening pooled and/or individual tomato plants. The tomato plants of the present invention exhibit reduced PG enzyme activity and fruit that soften more slowly post harvest without having the inclusion of foreign nucleic acids in their genomes.

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Seattle, WA, United States of America

Susan R Hurst