Ciba Corning Diagnostics Corp.

Chiron Diagnostics Corporation

Steve C Chang

Say-Jong Law

Stephen A Palmacci

Roger S Cubicciotti

Thomas E Miller

Uri Piran

William J Riordan

Elizabeth K Krodel

Christine A Vitkauskas

Carol K Klukas

Edward M Sunshine

James W Peterson

A new method for immobilization of small molecules on solid supports via a macromolecular spacer has been developed. A protein or another macromolecule is first immobilized in an aqueous medium, and the solid support is than washed with an organic solvent. The small molecule is coupled in an organic medium, followed by organic medium washes. The new solid phases are useful for affinity purifications, immunoassays and other binding assays, and for selection of binders by panning procedures.

Conjugation of ligand to immobilized protein in organic solvent

This invention is directed to the novel assay methods utilizing nucleophilic polysubstituted aryl acridinium ester conjugates as the tracers. Conjugates prepared by covalent coupling of novel nucleophilic polysubstituted aryl acridinium esters with biological compounds including small organic molecules such as Vitamin B12, folate, cortisol, estradiol, and thromboxane B2, were found useful in the development of highly sensitive assays for the analytes of diagnostic interest.

Nucleophilic polysubstituted aryl acridinium ester conjugates uses

The present invention relates to novel acridinium esters which are useful as luminescent labels in specific binding assays such as immunoassays or nucleic acid hybridization assays. More particularly, polysubstituted aryl acridinium esters are highly stable labels for use in a chemiluminescent immunoassay.

Polysubstituted aryl acridinium esters

A method for releasing encapsulated marker molecules from within liposomes comprises contracting the liposomes with an effective amount of a water-miscible alcohol. The chemiluminescence of acridinium esters is enhanced by oxidizing the acridinium ester in the presence of an effective amount of a water-miscible alcohol.

Method for enhancing signal release from liposomes using water-miscible

A method for enhancing the chemiluminescent signal of an acridinium ester in a chemiluminescent reaction which comprises oxidizing the acridinium ester in the presence of an enhancer selected from the group consisting of: (a) a cationic surfactant; (b) a nonionic surfactant; and (c) a sulfated primary alcohol.

Method for enhancement of chemiluminescence

A method for determining thyroxine uptake of a sample serum comprising: (a) incubating the sample serum with solid phase thyroxine and labeled anti-TBG antibody; (b) separating the solid phase from the unbound labeled antibody; (c) measuring the amount of label associated with the solid phase; and (d) calculating the thyroxide uptake ratio of the sample serum by relating the measurement of step (c) to a measurement of a reference serum.

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Franklin, MA, United States of America

Steve C Chang