Sepracor, Inc.

Stephen E. Zale

James L. O'connor

Randal A. Goffe

Stephen B. Kessler

Stephen E Zale

Clark K Colton

Randal A Goffe

Stephen B Kessler

James L O'connor

An affinity support having an improved capacity for binding target compounds. The support includes an immobilized modified ligand of increased molecular weight. The molecular weight of the ligand is increased via the action of condensation reagents or crosslinkers prior to immobilization. The affinity support is useful in affinity separations of proteins and other biomolecules from complex, biologically-derived fluids.

High-capacity affinity supports and methods for the preparation and use

This invention relates to methods and apparatus for carrying out affinity separations of proteins under conditions optimized for recovering biologically active protein. The method utilizes a membrane-bound ligand first to capture and separate a ligate from a fluid mixture and subsequently to release said ligate in purified form. The present methods enhance the yield of active ligate recovered in the process. The invention has particular relevance to the recovery and purification of proteins from mixtures.

Method and apparatus for eluting proteins in affinity membrane process

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Uxbridge, MA, United States of America

Stephen E Zale