Icon Genetics Ag

Icon Genetics Gmbh

Stefan Mühlbauer

Stefan Herz

Hans-Ulrich Koop

Christian Eibl

Sebastian Klaus

Fong-Chin Huang

Yuri Gleba

Victor Klimyuk

Sylvestre Marillonnet

Stefan Werner

Carola Engler

Timothy J Golds

Timothy James Golds

A process of producing a protein of interest by expression of said protein of interest from a sequence of interest in a plant or in plant leaves, comprising: (a) transfecting said plant or said plant leaves by infiltrating said plant or said plant leaves with anstrain in the presence of a complementing factor, saidstrain containing in T-DNA a heterologous DNA sequence having a sequence portion encoding a replicon, wherein said sequence encoding a replicon contains sequences necessary for replicon function of said replicon, said sequences being derived from a plant virus, and said sequence of interest to be expressed from said replicon, (b) optionally isolating said protein of interest from said plant or said plant leaves infiltrated in step (a), wherein saidstrain is provided with a first genetic modification rendering saidstrain defective for transfecting organisms with said T-DNA in the absence of said complementing factor.

Biologically safe transient protein expression in plants

A process of generating transgenic plants or plant cells transformed on their plastome and being devoid of a selection marker, comprising the following steps: (a) transforming plastids of a plant or plant cell with a DNA comprising: (i) a nucleotide sequence conferring replication of said DNA in a plant cell, (ii) at least one sequence of interest, (iii) sequences flanking said at least one sequence of interest necessary for stable integration of said at least one sequence of interest into the plastid genome, and (iv) a selection marker outside of said sequences flanking said sequence(s) of interest; (b) allowing integration of said at least one sequence of interest into the plastome in the presence of selective pressure; (c) allowing loss of said selection marker sequence by releasing selective pressure; and (d) recovering cells and/or plants being genetically transformed on their plastomes and being devoid of said selection marker.

Process of generating transplastomic plants or plant cells devoid of a selection marker

A process for producing multicellular plants, plant organs or plant tissues transformed on their plastome by the following steps is provided: (a) altering or disrupting the function of a gene in a plastid genome for producing a selectable or recognizable phenotype; (b) separating or selecting plants or cells having plastids expressing said phenotype; (c) transforming said plastid genome of said separated or selected plant, plant organ or plant tissue with at least one transformation vector having a restoring sequence capable of restoring said function; and (d) separating or selecting said transformed plant, plant organ or plant tissue having plastids expressing said restored function.

Processes and vectors for plastid transformation of higher plants

Growing community of inventors

Freising, Germany

Stefan Mühlbauer