Becton Dickinson and Company Limited

Pharmingen

Stefan Gruenwald

Zbigniew Darzynkiewicz

Frank Traganos

Gloria Juan

Craig Monell

Bi-Ching Sang

Methods, reagents, and kits are provided that permit flow cytometric determination of the phosphorylation status of retinoblastoma susceptibility gene protein (pRB) in individual cells. Methods are described that permit the hypophosphorylated, active, form of pRB to be measured either as an absolute quantity or as a proportion of total cellular pRB. Further described are methods that permit pRB phosphorylation status to be correlated with cell cycle phase and with protein components of the cell cycle. Screening of chemical compounds for antiproliferative and antineoplastic activity using the flow cytometric assays is demonstrated. Reagent kits that facilitate the subject methods are also provided.

Detection of protein conformations in single cells

Flow cytometric methods for the concurrent detection of discrete functional conformations of PRB in single cells

A monoclonal antibody which specifically binds with an E2A/pbx1 fusion epitope.

E2A/pbx1 fusion protein specific monoclonal antibodies

Growing community of inventors

Encinitas, CA, United States of America

Stefan Gruenwald