Cpc International, Inc.

Soichiro Ushiro

Shigehiro Enokizono

Masaki Tamura

Henry H Nonaka

Shiro Hasagawa

An improved process is provided for conversion of crude starch-containing materials such as broken-polished rice and corn grits to starch hydrolyzates and high fructose syrups. The process employs short steeping and milling steps before enzymatic liquefaction and saccharification of the starch. The process causes a minimum solubilization of protein giving readily purified products.

Process for making starch hydrolyzates and high fructose syrups

An immobilized glucose isomerase is prepared by treating an aqueous suspension of cells of a glucose isomerase producing microorganism with a non-ionic surfactant that solubilizes glucose isomerase contained by the cells without solubilizing polysaccharides in the cells, separating the cells from the aqueous suspension, and adsorbing glucose isomerase from the resultant solution onto an ion exchange resin. By this process, contaminating polysaccharides are eliminated that inhibit adsorption of glucose isomerase on the ion exchange resin.

Production of immobilized glucose isomerase

When immobilizing enzymes such as glucose isomerase by adsorption on a carrier such as an ion exchange resin, the enzyme prior to adsorption on the carrier is separated from a high molecular weight, non-dialyzable polysaccharide which inhibits adsorption of the enzyme to the carrier.

Immobilized enzymes

Glucose isomerase is immobilized in an active form by adsorbing the glucose isomerase onto a colloidal silica. The enzyme is contacted with the colloidal silica and the resulting composite solidified by freezing. Optionally, the composite may be gelatinized prior to freezing. The composite is then used for the isomerization of glucose to fructose.

Process for immobilizing glucose isomerase and a method of continuous

Process for immobilizing glucose isomerase

The continuous isomerization of dextrose to levulose is efficiently conducted in a fixed bed reactor, whose bed is charged with immobilized dextrose isomerase, by periodically adding fresh isomerase to the feed liquor. The carrier, on which the isomerase is immobilized, binds the fresh isomerase, so that enzyme activity is maintained in the reactor at an effective level, without interrupting operations.

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Kokubunji, Japan

Soichiro Ushiro