Iowa State University Research Foundation Inc

Omegachea

Shivani Garg

Basil J Nikolau

Marna Yandeau-Nelson

Huanan Jin

Xiaochen Yu

Ludmila Rizhsky

A mutant, which does not express a functional KASIIIA and/or KASIIIB, and method of making; a mutant, which does not express a functional PhaC1, PhaC2, and/or PhaC3, and method of making; method of characterizing substrate specificity of KASIII; method of making mutant KASIII with altered substrate specificity and/or altered level of activity and nucleic acid, vector, host cell/organism, and mutant KASIII; an in vitro, high-throughput spectrophotometric method of assaying KASIII activity; and materials and methods for using KASIII for production of bi-functional fatty acids and the materials so produced.

Materials and methods for characterizing and using a 3-ketoacyl-acyl carrier protein (ACP) synthase III (KASIII) for production of bi-functional fatty acids

A method of producing bi-functional fatty acids comprising introducing into a host cell or organism, which comprises one or more &#x3c9;- or &#x3c9;-1 functionalized acyl-CoAs, and expressing therein a KASIII, which can use one or more of the &#x3c9;- or &#x3c9;-1 functionalized acyl-CoAs as a substrate; a method of producing a &#x3c9;-1 hydroxy branched fatty acid, a &#x3c9;-1 branched fatty acid, or a combination thereof by culturing a mutant, which does not express a functional KASIII from the endogenous fabH gene and expresses a phaA and a phaB and a functional exogenous KASIII; and a mutant, a method of making the mutant, a culture comprising the mutant, and a composition comprising &#x3c9;-1 hydroxy branched fatty acids, a &#x3c9;-1 branched fatty acids, or a combination thereof obtained from the culture.

Materials and methods for production of bi-functional fatty acids in recombinant bacteria

Materials and methods for characterizing and using KASIII for production of bi-functional fatty acids

Growing community of inventors

Ames, IA, United States of America

Shivani Garg