Kyoto University

Dainippon Sumitomo Pharma Co., Ltd.

Other

National Institute of Advanced Industrial Science and Technology

Japan Biological Informatics Consortium

The University of Tokyo

Keio University

Shinya Yamanaka

Kazutoshi Takahashi

Keisuke Okita

Yoshinori Yoshida

Mari Ohnuki

Michiyo Koyanagi

Masato Nakagawa

Koji Tanabe

Naoki Goshima

Yoshifumi Kawamura

Takayuki Tanaka

Momoko Maekawa

Hiromi Mochizuki

Eiko Kaiho

Koji Eto

Hideyuki Okano

Hiromitsu Nakauchi

Norio Nakatsuji

Tatsutoshi Nakahata

Motonari Uesugi

Megumu Saito

Naoya Takayama

Kenji Miki

Yohei Okada

Kazumitsu Ueda

Masaya Nakamura

Hirofumi Suemori

Eihachiro Kawase

Hidekazu Nishiki-I

Yuto Fujibayashi

Masatoshi Nishizawa

Kyoko Miura

Shunsuke Funakoshi

Hong Hyenjong

Hidaka Yokota

Nao Hirata

Asako Murata

Young-Tae Chang

Kaori Yamauchi

Osahiko Tsuji

The invention provides an in vitro method of improving the efficiency of establishment of induced pluripotent stem (iPS) cells. The method comprises contacting an isolated somatic cell being reprogrammed into an iPS cell with an inhibitor of p53 function. The invention also provides an in vitro method of producing iPS cells. The method comprises bringing (a) nuclear reprogramming substances or nucleic acids encoding the nuclear reprogramming substances and (b) an inhibitor of p53 function into contact with a somatic cell.

Method of making induced pluripotent stem cells using p53 inhibitors

The present invention provides a production method of iPS cell, including a step of introducing the following (1) and (2):

Highly efficient method for establishing induced pluripotent stem cell

The present invention provides a method for screening for iPS cells exhibiting differentiation resistance using a marker identified as lincRNA or mRNA that is specifically expressed in an iPS cell line exhibiting differentiation resistance, and such markers.

Method for screening induced pluripotent stem cells

A method for producing hematopoietic stem cells and/or hematopoietic progenitor cells from pluripotent stem cells is described. The method includes a step of culturing pluripotent stem cells in the presence of IGF2. A method is described for selecting an induced pluripotent stem cell(s) having high capacity to differentiate into hematopoietic stem cells and/or hematopoietic progenitor cells, or into blood cells, based on the expression level(s) of one or more genes such as TRIM58, CTSF, FAM19A5, and TCERG1L genes, or on the DNA methylation state(s) of the TRIM58, CSMD1, and/or FAM19A5 gene(s).

Induced pluripotent stem cell selection method and method for inducing differentiation to blood cells

The present invention provides a method for producing or detecting cardiomyocytes by extracting/detecting cardiomyocytes from a cell population which includes cardiomyocytes using, as an index, positivity of NCAM1, SSEA3, SSEA4 and/or CD340.

Cardiomyocyte marker

The present invention provides a method of improving iPS cell establishment efficiency, comprising contacting a protein involved in primitive streak (PrS) formation, preferably Foxh1, or a nucleic acid that encodes the same with a somatic cell in a nuclear reprogramming step. Also provided is a method of producing an iPS cell, comprising contacting the protein involved in PrS formation or a nucleic acid that encodes the same, and nuclear reprogramming substance(s) with a somatic cell.

Method of efficiently establishing induced pluripotent stem cells

The present invention provides a method for efficiently producing cardiomyocytes from pluripotent stem cells, which method comprises the steps of dissociating embryoid bodies obtained during the production process, and allowing reaggregation of the resulting cells to allow formation of embryoid bodies.

Method of efficiently inducing cardiomyocytes

This relates to a method of preparing induced pluripotent stem cells, comprising a nuclear reprogramming step with a nuclear reprogramming factor in the presence of miRNA, wherein said miRNA has a property of providing a higher nuclear reprogramming efficiency in the presence of said miRNA than in the absence thereof.

Efficient method for nuclear reprogramming

Provided is a method of improving the efficiency of iPS cell establishment, comprising bringing one or more factors selected from the group consisting of proteins belonging to cyclin D family and nucleic acids that encode the same into contact with a somatic cell, in the step of nuclear reprogramming of the somatic cell. Also provided are a method of producing an iPS cell comprising the step of bringing the factor(s) and nuclear reprogramming substance(s) into contact with a somatic cell, an iPS cell comprising a nucleic acid that encodes a protein belonging to cyclin D family that can be obtained by the method of producing an iPS cell, and a method of somatic cell production by forcing the iPS cell to differentiate.

The present invention provides a method for screening drugs for suppressing inflammasome activity, using macrophages derived from induced pluripotent stem cells (iPS cells) having mutant NLRP3 gene.

Method for screening drugs for suppressing inflammasome activity

Provided is a method of improving the efficiency of establishment of induced pluripotent stem cells, comprising culturing somatic cells under hypoxic conditions in the step of nuclear reprogramming thereof.

Methods of efficiently establishing induced pluripotent stem cells under hypoxic conditions

The invention provides a method of improving the efficiency of establishment of induced pluripotent stem cells by increasing, in a nuclear reprogramming step of somatic cell, the level of activated form of one or more proteins selected from the group consisting of Ras family members, PI3 kinase, RalGEF, Raf, AKT family members, Rheb, TCL1 and S6K. The invention also provides a method of producing induced pluripotent stem cells by contacting a somatic cell with a nuclear reprogramming substance and one or more of such proteins and nucleic acids that encode such proteins. The invention further provides an induced pluripotent stem cell that has an exogenous nucleic acid encoding such a protein, as well as agents for use in the aforesaid methods.

Efficient method for establishing induced pluripotent stem cells

A method of producing an induced pluripotent stem cell includes introducing into a somatic cell one or more non-viral expression vectors. The vectors include one or more of an Oct family gene, a Klf family gene, a Sox family gene, a Myc family gene, a Lin family gene, and Nanog gene. The somatic cell is then cultured in a medium that supports pluripotent stem cells. At least a portion of the one or more introduced non-viral expression vectors is not substantially integrated in the chromosome.

Method of making induced pluripotent stem cells

Growing community of inventors

Kyoto, Japan

Shinya Yamanaka