Shionogi Sieyaku Kabushiki Kaisha

Mitsubishi Chemical Industries Limited

Mitsubishi Kasei Corporation

Merrell Pharmaceuticals, Inc.

Shionogi Seiyaku Kabushiki Kaisha

Shigetada Nakanishi

Kazuwa Nakao

Hiroo Imura

Yutaka Teranishi

Kazuhiro Takahashi

Naomi Kitamura

Kenji Nagahari

Stephen H Buck

Ken Takamatsu

Rie Matsui

Tatsurou Shibiu

Henry J Yamamura

Michiru Kamizono

William R Roeske

Alden Keith Henderson

Tatsurou Shibui

Josephine Yuen-Wai Lai

DNA encoding an endothelin receptor shown in SEQ ID NO: 1 or 2 in the Sequence Listing is isolated from cDNA which is prepared from poly(A)RNA derived from a human placenta. In addition, an expression vector containing the DNA and a transformant containing the expression vector are obtained. An endothelin receptor is obtained by culturing this transformant. A receptor shown in SEQ ID NO: 1 is an ET-receptor which has a high affinity for endothelins 1 and 2, especially for the endothelin 1. A receptor shown in SEQ ID NO: 2 is an ET-receptor which has an affinity for endothelins 1, 2 and 3 with no selectivity.

Human endothelin receptor

DNA encoding an endothelin receptor shown in SEQ ID NO: 1 or 2 in the Sequence Listing is isolated from cDNA which is prepared from poly(A) RNA derived from a human placenta. In addition, an expression vector containing the DNA and a transformant containing the expression vector are obtained. An endothelin receptor is obtained by culturing this transformant. A receptor shown in SEQ ID NO: 1 is an ET -receptor which has a high affinity for endothelins 1 and 2, especially for the endothelin 1. A receptor shown in SEQ ID NO: 2 is an ET -receptor which has an affinity for endothelins 1, 2 and 3 with no selectivity.

DNA encoding an endothelin receptor shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4. In the Sequence Listing is isolated from cDNA which is prepared from poly(A),RNA derived from a human placenta. In addition, an expression vector containing the DNA and a transformant containing the expression vector are obtained. An endothelin receptor is obtained by culturing this transformant. A receptor shown in SEQ ID NO: 1 and SEQ ID NO: 2 is an ET,-receptor which has a high affinity for endothelins 1 and 2, especially for the endothelin 1. A receptor shown in SEQ ID NO: 3 and SEQ ID NO: 4 is an ET,-receptor which has an affinity for endothelins 1, 2 and 3 with no selectivity.

A method for producing high level expression of neuropeptide receptors and stable cell lines useful therein. This method involves culturing a cell line containing amplified copies of a cDNA sequence encoding a neuropeptide receptor containing an expression control sequence taken from the .beta.-actin transcriptional control element.

Neurokinin receptor cell lines

Provided herein are a DNA fragment which contains a base sequence coding for a non-A non-B hepatitis-specific antigenic protein occurring in cells of the liver affected with non-A non-B hepatitis, an expression vector in which said DNA fragment is inserted into a cloning site present downstream from a promoter thereof, a transformant obtained by introducing said expression vector into a host, and a process for producing said antigenic protein which comprises providing said expression vector, transforming a host with said expression vector, culturing the transforming host and collecting the protein produced therein.

DNA fragments coding for antigens specific to non-A non-B hepatitis,

A recombinant DNA vector containing a DNA sequence which encodes a peptide having the physiological properties of cardionatrin ad cardiodilatin.

Expression vectors encoding cardionatrin and cardiodilatin

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Kyoto, Japan

Shigetada Nakanishi