The General Hospital Corporation

Shannon Tessier

Mehmet Toner

Shannon Stott

Mustafa Korkut Uygun

Lindong Weng

Keith Wong

Rebecca Sandlin

Renier Johan De Vries

Casie A Pendexter

Stephanie E J Cronin

Reinier Johan De Vries

This disclosure is related to methods of preserving biological samples such as organs, and tissue. The present disclosure relates to methods of subzero preservation of biological tissue samples, such as entire organs from mammals, e.g., humans. The present disclosure is based, at least in part, on the discovery that biological tissue samples can be supercooled while minimizing formation of ice crystals by reducing liquid-air interfaces and lowering the melting point of the tissue samples, e.g., organs, or liquid in the tissue samples by use of cryoprotective agents while ensuring uniform distribution of cryoprotective agents throughout the biological sample by using improved perfusion techniques.

Cryopreservation of tissues and organs

This disclosure is related to methods of preserving biological samples, organs, and organisms. This disclosure is based, at least in part, on the discovery that in the presence of heterogenous extracellular ice, endothelial cells have improved attachment and viability after preservation, and particularly by uniformly nucleating ice across the microvasculature using ice nucleating agents, the chance of intracelluar ice formation can be significantly reduced.

High subzero cryopreservation

Methods for stabilizing blood samples, e.g., clinical blood samples, for storage or transportation before use.

Stabilization of whole blood samples

This disclosure relates to ice nucleation formulations for cryopreservation and stabilization of biologics, and methods of use thereof.

Ice nucleation formulations for cryopreservation and stabilization of biologics

Methods for stabilizing blood samples, e.g., clinical blood samples, for storage or transportation before use. Exemplary applications include but are not limited to enrichment of leukocyte subtypes such as T-cells or neutrophils for cytokine and immuno-assays; isolation of progenitor cells from cord blood or peripheral blood for transplantation; isolation of fetal cells from the maternal blood for diagnosis; and sorting of circulating tumor cells for cancer detection and therapy.

Growing community of inventors

Cambridge, MA, United States of America

Shannon Tessier