Ajinomoto Co., Ltd.

Other

Sergei Vladimirovich Mashko

Vera Georgievna Doroshenko

Aleksandra Yurievna Skorokhodova

Mikhail Markovich Gusyatiner

Elvira Borisovna Voroshilova

Yury Ivanovich Kozlov

Irina Vladimirovna Biryukova

Yoshihiko Hara

Konstantin Vyacheslavovich Rybak

Takuji Ueda

Tatyana Viktorovna Leonova

Elena Vitalievna Klyachko

Ekaterina Aleksandrovna Slivinskaya

Ekaterina Alekseevna Savrasova

Vitaliy Arkadyevich Livshits

Larisa Gotlibovna Airikh

Aleksandr Aleksandrovich Krylov

Valeriy Zavenovich Akhverdian

Hisashi Yasueda

Alla Valentinovna Belareva

Shuhei Hashiro

Maria Viacheslavovna Vitushkina

Andrey Yurievich Gulevich

Danila Vadimovich Zimenkov

Mayu Mitsuhashi

Zhanna Iosifovna Katashkina

Vladimir Yurievich Ermishev

Yuliya Sergeevna Lobanova

Vitaly Arkadievich Livshits

Natalia Viktorovna Stoynova

Nelli Isaakovna Zhdanova

Evgeny Moiseevich Khurges

Nikolai Kazimirovich Yankovsky

Jury I Kozlov

Raisa Alexandrovna Arsatiants

Natalia Pavlovna Zakataeva

Rustem Saidovich Shakulov

Valery Zavenovich Akhverdian

Joanna Yosifovna Katashkina

Maria Grigorievna Lunts

Vladimir G Debabov

Lirina Valerievna Ivanovskaya

Svetlana Aleksandrovna Fomina

Ljudmila F Kozyreva

Evgeniya Aleksandrovna Malykh

Tatyana Aleksandrovna Gaydenko

Natalya Igorevna Minaeva

Aleksandr Dmitrievich Kivero

Mikhail N Rozinov

Boris A Rebentish

Vera N Moshentseva

Maria Kharchenko

Aleksander Krylov

Ekaterina Andrianova

Mikhail Baboshin

Lyubov Golubeva

Ekaterina Kovaleva

Mikhail Shupletsov

The present invention provides a method for producing a target substance, the biosynthetic pathway of which is ATP-dependent, for example, amino acids, nucleosides, nucleotides, isoprenoids, and peptides, by fermentation of a bacterium which has been modified to overexpress a gene encoding a protein having H-translocating membrane-bound pyrophosphatase activity, for example, the hppA gene native toor a variant thereof.

Method for producing target substance by bacterial fermentation

A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.

Method for producing RNA

There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.

Method for producing L-amino acids using bacteria of the enterobacteriaceae family

The present invention provides a method for constructing recombinant translationally coupled operons, a method for producing useful metabolites using the bacterium containing the coupled operons, and a method for monitoring gene expression.

Method for constructing an operon containing translationally coupled genes

An expression control sequence which controls expression of a target gene linked downstream of the expression control sequence depending on an intracellular concentration of an amino acid, wherein in a bacterial cell which harbors a DNA construct comprising the expression control sequence, a promoter linked upstream of the expression control sequence and the target gene linked downstream of the expression control sequence, frequency of termination in the expression control sequence, of transcription starting from the promoter is lowered by increase of an intracellular concentration of an amino acid, whereby expression of the target gene increases.

Expression control sequence

A method for producing lower alkyl ester of &#x3b1;-L-aspartyl-L-phenylalanine by cultivating a recombinantbacterium that has the ability to produce and accumulate an aromatic L-amino acid according in a culture medium to produce and accumulate L-phenylalanine in the medium and synthesizing lower alkyl ester of &#x3b1;-L-aspartyl-L-phenylalanine from the aspartic acid or its derivative and the obtained L-phenylalanine.

Method for producing a lower alkyl ester of &#x3b1;-L-aspartyl-L-phenylalanine

Method for producing L-amino acids using bacteria of the Enterobacteriaceae family

The present invention provides a method for producing an aromatic L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genusor, which has been modified to attenuate expression of the ydiN gene, the ydiB gene, or both.

Method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family with attenuated expression of the ydiN gene or the ydiB gene or combination thereof

The present invention relates to a method for producing L-amino acid, such as L-phenylalanine and L-tryptophan, is provided using bacterium belonging to the genuswherein the L-amino acid productivity of said bacterium is enhanced by enhancing an activity of protein encoded by the yddG gene from, wherein said protein has an activity to make said bacterium resistant to L-phenylalanine, a phenylalanine analogue, or a tryptophan analogue. The present invention further relates to a method for producing lower alkyl ester of &#x3b1;-L-aspartyl-L-phenylalanine.

[object Object]

A method is provided for obtaining an L-amino acid or nucleic acid-producing bacterium belonging to the genuswith an optimized level of expression of the gene which influences the distribution of carbon flow, such as the sucAB genes, comprising introducing into the chromosome of the bacterium a set of in vitro constructed DNA fragments which contain regulatory elements for gene expression instead of the native elements of the regulatory region of the gene, and selecting the colonies with increased L-amino acid productivity. Also, a method is provided for producing an L-amino acid, such as L-glutamic acid, L-proline, L-arginine, L-glutamine, L-leucine, using the bacterium with an optimized level of expression of the sucAB gene.

Method for producing an L-amino acid using a bacterium with an optimized level of gene expression

Amino acids such as threonine, homoserine, isoleucine, lysine, valine and tryptophan are produced using a bacterium belonging to the genuswhich has been constructed from a sucrose non-assimilative strain belonging to the genusand which harbors sucrose non-PTS or PTS genes and has an ability to produce the amino acid.

A method for constructing strains which produce aminoacids comprising combining of a DNA chromosome fragment of a donor microorganism containing genes controlling the synthesis of a selected aminoacid and having a mutation destroying the negative regulation of the synthesis of this aminoacid with a vector DNA molecule to form a hybrid DNA molecule. Use is made of a vector DNA molecule capable of providing amplification of the hybrid DNA molecule. The resulting hybrid DNA molecule is used for transforming cells of the recipient strain having the mutation blocking the synthesis of the selected aminoacid in this strain and the mutation partly blocking the related step of metabolism of this aminoacid to yield the strain capable of increased productivity of the selected aminoacid.

Method for preparing strains which produce aminoacids

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Moscow, Russia

Sergei Vladimirovich Mashko