University Patents, Inc.

Beckman Instruments, Inc.

Serge L Beaucage

Marvin H Caruthers

Jang B Rampal

Paramesewara M Reddy

Method for substituting a substitution compound for the hydrogen of a support bound primary hydroxyl group. The support bound primary hydroxyl group is treated in an organic, substantially anhydrous solvent in the presence of a tertiary amine base and the substitution compound. The substitution compound has a positive moiety and a negative moiety. The positive moiety is selected from the group consisting of trityl, pixyl, and derivatives thereof. The reagent is selected from the group consisting of tetraalkylammonium salts, inorganic salts, and mixtures thereof. Each reagent has a cation and an anion moiety. The cation moiety of the reagent is more electropositive than the positive moiety of the substitution compound. This relationship enables the reagent to attract the negative moiety of the substitution compound.

Process for terminal substituting of a polynucleotide

A new class of nucleoside phosphoramidites which are relatively stable to permit isolation thereof and storage at room temperature. The phosphoramidites are derivatives of saturated secondary amines.

Process for oligonucleo tide synthesis using phosphormidite intermediates

Phosphoramidite nucleoside compounds

Phosphoramidite compounds and processes

Growing community of inventors

Mountain View, CA, United States of America

Serge L Beaucage