Hayashibara Co., Ltd.

Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo

Seisuke Izawa

Takashi Shibuya

Shigeharu Fukuda

Toshio Miyake

Tomoyuki Nishimoto

The invention provides a process for enabling the production of a particulate composition containing anhydrous crystalline ascorbic acid 2-glucoside that does not significantly cake even when the production yield of ascorbic acid 2-glucoside does not reach 35% by weight. The process for producing a particulate composition containing anhydrous crystalline ascorbic acid 2-glucoside, which comprises allowing a CGTase to act on a solution containing either liquefied starch or dextrin and L-ascorbic acid and then allowing a glucoamylase to act on the resulting solution to obtain a solution with an ascorbic acid 2-glucoside production yield of at least 27%, purifying the obtained solution to increase the ascorbic acid 2-glucoside content to a level of over 86% by weight, precipitating anhydrous crystalline ascorbic acid 2-glucoside by a controlled cooling method or pseudo-controlled cooling method, collecting the precipitated anhydrous crystalline ascorbic acid 2-glucoside, and ageing and drying the collected anhydrous crystalline ascorbic acid 2-glucoside.

Process for producing a particulate composition comprising an hydrous crystalline 2-o-alpha-d-glucosyl-ascorbic acid

A process for enabling the production of a particulate composition containing crystalline trehalose dihydrate is provided. Including allowing an &#x3b1;-glycosyltrehalose-forming enzyme to act on liquefied starch derived from a microorganism of the genusand a trehalose-releasing enzyme derived from a microorganism of the genusalong with a starch debranching enzyme and a cyclomaltodextrin glucanotransferase; allowing glucoamylase to act on the resulting mixture to obtain a saccharide solution containing &#x3b1;,&#x3b1;-trehalose; precipitating crystalline &#x3b1;,&#x3b1;-trehalose dihydrate from the above saccharide solution; collecting the precipitated crystalline &#x3b1;,&#x3b1;-trehalose dihydrate by a centrifuge; and ageing and drying the collected crystals. Cyclomaltodextrin glucanotransferase derived from a microorganism of the genusor a mutant enzyme thereof is used to increase the &#x3b1;,&#x3b1;-trehalose content in the saccharide solution to over 86.0% by weight, on a dry solid basis, without passing through a fractionation step by column chromatography.

Process for producing a particulate composition comprising crystalline &#x3b1;,&#x3b1;-trehalose di-hydrate

Process for producing a particulate composition comprising crystalline alpha, alpha-trehalose di-hydrate

Process for producing a particulate composition comprising an hydrous crystalline 2-O-&#x3b1;-D-glucosyl-L-ascorbic acid

Process for producing a particulate composition comprising crystalline trehalose dihydrate

Process for producing a particulate composition comprising anhydrous crystalline 2-O-alpha-D-glucosyl-L-ascorbic acid

The present invention has objects to provide a novel crystalline 2-O-&#x3b1;-glucosyl-L-ascorbic acid and its production process and uses, and solves the above objects by providing hydrous crystalline 2-O-&#x3b1;-glucosyl-L-ascorbic acid, a particulate composition containing hydrous crystalline 2-O-&#x3b1;-glucosyl-L-ascorbic acid, and their production processes and uses.

Hydrous crystalline 2-O-&#x3b1;-D-glucosyl-L-ascorbic acid, particulate composition comprising the same, their preparation and uses

The present invention aims to provide a particulate composition containing anhydrous crystalline 2-O-&#x3b1;-D-glucosyl-L-ascorbic acid having a significantly, hardly solidifiable property compared to conventional ones in a grade for use in quasi-drugs; a process for producing the same; and uses thereof. The present invention solves the above object by providing a particulate composition containing anhydrous crystalline 2-O-&#x3b1;-D-glucosyl-L-ascorbic in an amount of over 98.0% by weight but less than 99.9% by weight, on a dry solid basis; or a degree of crystallinity of 90% or higher for anhydrous crystalline 2-O-&#x3b1;-D-glucosyl-L-ascorbic acid; and by providing a process for producing the same and uses thereof.

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Okayama, Japan

Seisuke Izawa