Ajinomoto Co., Ltd.

Seiko Hirano

Hisao Ito

Kazuhiko Matsui

Jun Nakamura

Tsuyoshi Nakamatsu

Mika Moriya

Osamu Kurahashi

Shinichi Sugimoto

Yoshihiko Hara

Yoshio Kawahara

Hiroshi Izui

Eiichiro Kimura

Gen Nonaka

Tsuyoshi Osumi

Yumi Matsuzaki

Naoki Akiyoshi

Kanae Nakamura

Hisashi Yasueda

Masakazu Sugimoto

Atsushi Hayakawa

Masako Izui

Kazuyuki Hayashi

Kenzo Yokozeki

Hiroshi Matsui

Keita Fukui

Yasuhiko Yoshihara

Yoshiya Gunji

Takayuki Asahara

Eiichi Nakano

Mikiko Yamaguchi

Hitoshi Takayashiki

Reiko Hirai

A method for producing an L-amino acid of glutamate family such as L-glutamic acid is provided. An L-amino acid of glutamate family is produced by culturing a coryneform bacterium having an ability for producing an L-amino acid of glutamate family, which has been modified so that the activity of an &#x3b1;-ketoglutaric acid (&#x3b1;-KG) uptake carrier is increased, in a medium, and collecting the L-amino acid of glutamate family from the medium.

Method for producing L-amino acid of glutamate family

A method for producing an L-amino acid is provided. An L-amino acid is produced by culturing a coryneform bacterium having an L-amino acid-producing ability, which is modified so that the activity of a phosphate transporter is increased, in a medium, and collecting the L-amino acid from the medium.

Method for producing L-amino acid using microorganism having increased phosphate transporter activity

A coryneform bacterium that is modified by using a yggB gene so that L-glutamic acid-producing ability is enhanced as compared to a non-modified strains is cultured in a medium to cause accumulation of L-glutamic acid in the medium or bacterial cells, and L-glutamic acid is collected from the medium or cells.

L-glutamic acid-producing microorganism and a method for producing L-glutamic acid

A method for producing L-glutamic acid by culturing a coryneform bacterium in which the gluX is inactivated in a medium to produce L-glutamic acid in the medium or cells, and collecting L-glutamic acid from the medium.

L-glutamic acid-producing bacterium and method for production of L-glutamic acid

A method for production of L-lysine is provided which includes the steps of cultivating a methanol-utilizing bacterium in a culture medium to produce and accumulate L-lysine in the culture medium and collecting the L-lysine from the culture medium, wherein the methanol-utilizing bacterium contains DNA encoding dihydrodipicolinate synthetase which is desensitized to feedback inhibition by L-lysine and DNA encoding a LysE protein that can enhance the excretion of L-lysine out of the methanol-utilizing bacterium, and the bacterium is modified so as to increase the intracellular activities of diaminopimelic acid dehydrogenase, diaminopimelic acid decarboxylase, dihydrodipicolinic acid reductase and aspartate-semialdehyde dehydrogenase.

Method for production of L-lysine using methanol-utilizing bacterium

A microorganism is provided which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and which has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH. Also provided is a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

Method for producing L-glutamic acid by fermentation accompanied by precipitation

A method for producing L-glutamic acid by culturing a coryneform bacterium which has L-glutamic acid producing ability and which has been modified so that expression of the fasR gene is enhanced in a medium to produce and accumulate L-glutamic acid in the medium or cells, and collecting L-glutamic acid from the medium or cells.

L-glutamic acid producing bacterium and a method for production of L-glutamic acid

L-Lysine is produced by culturing a methanol-utilizing bacterium which requires L-methionine for its growth and has an ability to produce L-lysine in a medium containing methanol as a main carbon source to produce and accumulate L-lysine in culture and collecting the L-lysine from the culture.

Method for producing L-lysine using methanol-utilizing bacterium

A coryneform bacterium which has an L-glutamic acid-producing ability and grows at least at the same growth rate as a non-mutated strain or a wild-type strain and has intracellular &#x3b1;-ketoglutarate dehydrogenase activity which is less than half that of the non-mutated or wild-type strain, and is obtained by introducing a mutation into a coding region or an expression control region of the chromosomal odhA gene encoding the E1o subunit of the &#x3b1;-ketoglutarate dehydrogenase complex.

A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from, preferably an enzyme that functions at a higher temperature compared with that of. PCR is performed by using the primers and chromosomal DNA ofas a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of

Genes for heat resistant enzymes of amino acid biosynthetic pathway derived from thermophilic coryneform bacteria

A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived frompreferably an enzyme that functions at a higher temperature compared with that ofPCR is performed by using the primers and chromosomal DNA ofas a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of

A microorganism which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH; and a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

Growing community of inventors

Kawasaki, Japan

Seiko Hirano