Itoham Foods Inc.

Other

Seiji Sato

Toshiyuki Kudo

Masaaki Kondo

Naohiko Higashikuni

Toyoo Nakamura

Shusaku Oka

Yoshiaki Itagaki

Shizuyo Sutou

Shigezo Udaka

Shigeaki Watanabe

Yoshihiro Waki

Hirotaka Yuki

The invention relates to a DNA that enables co-expression of a protein disulfide isomerase and a polypeptide having disulfide bonds. The invention further relates to a process of producing polypeptides using the DNA and a process of enhancing the efficiency of formation of correct disulfide bond in an expression system of a gene recombinant polypeptide having disulfide bonds.

Process for producing polypeptide having disulfide bond

This invention relates to a DNA encoding a fusion protein of formula (I)[Y]-[X1]-[B-chain]-[X2]-[Linker]-[X3]-[A-chain]&#x2003;&#x2003;(I)wherein

Methods of producing recombinant insulin from novel fused proteins

This invention relates to a DNA comprising a nucleotide sequence encoding a fusion protein, wherein the fusion protein comprises: a sequence of signal peptide for Bacillus cell wall protein (CWP); a tag sequence for separation and purification of the fusion protein; a linker sequence; a sequence for chemical or enzymatic cleavage; and an exogenous polypeptide sequence, the sequences being linked in order, the signal peptide, tag and linker being optional sequences; and wherein the nucleotide sequence encoding a fusion protein is ligated to 3&prime;-end of a nucleic acid sequence comprising a Bacillus promoter region; to a vector comprising the DNA; to a bacterium belonging to the genus Bacillus comprising the vector; and to a process for preparation of a useful polypeptide by culture of the bacterium.

DNAs encoding new fusion proteins and processes for preparing useful polypeptides through expression of the DNAs

The present invention provides PCR primers with which sexing of bovine embryos can be easily attained and provides a practical, rapid and reliable method for determining the sex of bovine embryos using these primers. The methods for determining the sex of the bovine embryos are characterized by discriminating PCR products which are obtained by amplifying specific DNA sequences by PCR with pairs of male-specific and gender-neutral primers. These primers are derived from DNAs which specifically hybridize to the bovine male genome and from DNAs which gender-neutrally hybridize to both bovine male and female genomes.

Sexing method of bovine embryos

Sexing method of bovine embyros

Growing community of inventors

Ibaraki, Japan

Seiji Sato