E.i. Dupont De Nemours and Company

Pioneer Hi-bred International, Inc.

Other

Sean J Coughlan

Rebecca E Cahoon

Edgar Benjamin Cahoon

Karlene H Butler

Craig E Hastings

Christopher Jay Scelonge

Dennis L Bidney

Lijuan Wang

Timothy George Helentjaris

George William Singletary

Paul C Anderson

Rudolf Jung

Mark E Williams

Dean Della Penna

Eva Collakova

Ronnie J Winfrey, Jr

Ron J Winfrey, Jr

David Hu

The invention provides isolated nucleic acids and their encoded polypeptides that alter tocol content in seeds. The invention further provides expression cassettes, host cells and transformed plants containing the nucleic acids. The present invention further provides methods for altering tocol content in seeds.

Compositions and methods for altering tocotrienol content

The invention provides isolated nucleic acids and their encoded polypeptides that alter tocotrienol content in seeds. The invention further provides expression cassettes, host cells and transformed plants containing the nucleic acids. The present invention further provides methods for altering tocotrienol content in seeds.

This invention relates to an isolated nucleic acid fragment encoding a vitamin E biosynthtetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the vitamin E biosynthtetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the vitamin E biosynthtetic enzyme in a transformed host cell.

Plant vitamin E biosynthetic enzymes

This invention relates to an isolated nucleic acid fragment encoding a vitamin E biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the vitamin E biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the vitamin E biosynthetic enzyme in a transformed host cell.

Plant vitamin e biosynthetic enzymes

The invention provides isolated nucleic acids and their encoded proteins that are involved in tocopherol or plastoquinone biosynthesis. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering phytyl/prenyltransferase protein content and/or composition of plants.

Phytyl/prenyltransferase nucleic acids, polypeptides and uses thereof

This invention relates to an isolated nucleic acid fragment encoding a carotenoid biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the carotenoid biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the carotenoid biosynthetic enzyme in a transformed host cell.

Carotenoid biosynthesis enzymes

Compositions and methods for altering the content of plant seeds are provided. The compositions comprise nucleotide sequences encoding the enzyme acetyl-CoA synthetase. Such compositions find use in increasing the biosynthesis of fatty acids and/or carotenoids in plants. By expressing the sequences utilizing seed-specific promoters, plant seed can be obtained having increased levels of oils, specialty oils, carotenoids, and amino acids.

Compositions and methods for altering an acetyl-CoA metabolic pathway of a plant

Antisense expression of a stearoyl-ACP desaturase gene in sunflower results in more than a four-fold increase in seed stearate, and can enhance palmitate content as well. Thus, sunflower oil containing over 40 percent saturated fatty acids can be produced, which is desirable in the context of various products, such as coating fat, margarine, soap and shortening.

Sunflower seeds with enhanced saturated fatty acid contents

The present invention provides methods of obtaining a greater than normal concentration of oil in the seed of a plant, the method comprising altering the carbon metabolism in the seed leading to an alteration in the partitioning of assimilates from starch biosynthesis to enhance the biosynthesis and accumulation of oil, preferably without significantly affecting the size of the storage organ in a way that adversely impacts the commercial value of the material. Seeds produced by the present methods are also provided.

Methods of producing high-oil seed by modification of starch levels

The present invention provides a composition and method for regulating expression of heterologous nucleotide sequences in a plant. The composition is a novel nucleic acid sequence for a seed-preferred promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the seed-preferred promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell.

Seed-preferred promoter

Isolated nucleic acid molecules are provided which encode calreticulin and calnexin. Also provided are vectors which are capable of expressing such nucleic acid molecules, host cells which contain such vectors, and polypeptides encoded by the afore-mentioned nucleic acids. In addition, nucleic acid molecules are provided which comprise calreticulin or calnexin promoters.

Growing community of inventors

Hockessin, DE, United States of America

Sean J Coughlan