Stemcell Technologies Canada Inc.

Sqz Biotechnologies Company

Scott Loughhead

Howard Bernstein

Armon R Sharei

Matthew Booty

Kelan Hlavaty

Maisam Dadgar

Jonathan B Gilbert

Tarek Abdeljawad

Finola Moore

Bu Wang

Carolyne Kelly Smith

Katarina Blagovic

Jason Murray

Defne Yarar

David Chirgwin

Emrah Ilker Ozay

Harry An

Amritha Ramakrishnan

Anita Venkitaraman

Katherine Seidl

The present invention provides methods for inducing tolerance and/or suppressing an immune response to an antigen by passing a cell suspension containing an anucleate cell through a constriction, wherein the constriction deforms the cell thereby causing a perturbation of the cell such that an antigen and/or tolerogenic factor enters the cell. In some embodiments, the anucleate cell is delivered to an individual and the antigen is delivered to and processed in a tolerogenic environment to induce tolerance and/or suppress an immune response to the antigen.

Intracellular delivery of biomolecules to induce tolerance

The present application provides formulations of modified peripheral blood mononuclear cells (PBMCs), wherein the formulation comprises: PBMCs comprising at least one antigen (e.g., a human papillomavirus (HPV) antigen) and a cryopreservation medium. In some embodiments, the PBMCs are conditioned by incubating the PBMC in the presence of an adjuvant.

Formulations of PBMCs

The present application provides peripheral blood mononuclear cells comprising an antigen, methods of manufacturing such PBMCs, and methods of using such PBMCs, such as for modulating an immune response in an individual. In some embodiments, the PBMCs are conditioned by incubating the PBMC in the presence of an adjuvant.

Delivery of biomolecules to PBMCs to modify an immune response

Growing community of inventors

Watertown, MA, United States of America

Scott Loughhead