Solazyme, Inc.

Corbion Biotech, Inc.

Terravia Holdings, Inc.

Checkerspot, Inc.

Algenist Holdings, Inc.

The Scripps Research Institute

Scott Franklin

George N Rudenko

Aravind Somanchi

Xinhua Zhao

Riyaz Bhat

Anthony G Day

Walter Gregory Rakitsky

Jeffrey L Moseley

Karen Espina

Janice Lau Wee

Penelope Chua

Anna Coragliotti

Harrison F Dillon

Donald Eugene Trimbur

Alejandro G Marangoni

Risha Lindig Bond

Chung-Soon Im

Diza Braksmayer

Geoffrey Brooks

Walt Rakitsky

David Davis

Jason Casolari

Wenhua Lu

Stephen M Decker

Felipe Arana Rodriguez

Daniel Malmrose

Shane A Brubaker

Matthew Shoa-Azar

Aren Ewing

John Piechocki

Leslie M Norris

Tina Huynh

Jon Wittenberg

Enrique Baliu

Matthew Sterbenz

Trung H Nguyen

Dan Elefant

Jeff Avila

Neal Anderson

Garrett Witmer

Charles Rand

Zoran S Petrovic

Stephen P Mayfield

Jian Hong

Dana Zdanis

Jasna Djonlagic

Mona Correa

Kevin Ward

Veronica Benites

Leon Parker

Milica Lovric Vukovic

Alejandro Marangoni

Lesile M Norris

Jeffrey Moseley

Harrison Fields Dillon

The invention provides novel microalgal food compositions comprising microalgal biomass that have been processed into flakes, powders and flours. The microalgal biomass of the invention is low in saturated fats, high in monounsaturated triglyceride oil and can be a good source of fiber. The invention also comprises microalgal biomass that is suitable as a vegetarian protein source and also as a good source of fiber. Novel methods of formulating food compositions with the microalgal biomass of the invention are also disclosed herein including beverages, baked goods, egg products, reduced fat foods and gluten-free foods. The provision of food compositions incorporating the microalgal biomass of the invention to a human have the further benefit of providing healthful ingredients while achieving levels of satiety sufficient to reduce further caloric intake. The invention also provides novel strains of microalgae that have been subject to non-transgenic methods of mutation sufficient to reduce the coloration of the biomass produced by the strains. Oil from the microalgal biomass can be extracted and is an edible oil that is heart-healthy. The novel microalgal biomass and oil therefrom can be manufactured from edible and inedible heterotrophic fermentation feedstocks, including corn starch, sugar cane, glycerol, and depolymerized cellulose that are purpose-grown or byproducts of existing agricultural processes from an extremely broad diversity of geographic regions.

Microalgal food compositions

This disclosure provides methods for the chemical modification of microbial derived triglyceride oils, use thereof in polyurethane chemistries, and incorporation thereof as a core material alone or as part of a wood core composite in the production of sporting goods equipment, including, for example, alpine skis, touring skis, cross country skis, approach skis, split boards, snowboards, and water skis.

Uses of microbial derived materials in polymer applications

Provided herein are conglomerate resin compositions, methods for the creation of conglomerate resins made from waste stream materials, and incorporation of conglomerate resins in the construction of sporting goods equipment, including, for example, alpine skis, touring skis, cross country skis, approach skis, split boards, snowboards, and water skis.

Recycled polyurethane formulations

Compositions of microalgae-derived flour are disclosed from multiple genera, species, and strains of edible microalgae. Microalgae used in the invention are free of algal toxins and contain varying levels of primarily monounsaturated triglyceride oil. Flours disclosed herein are formulated as free flowing blendable powders, mixed food ingredients, oxidation stabilized, homogenized and micronized, and combinations therein. Flours disclosed herein also form self stabilizing emulsions in slurries with manageable viscosities. Innovative methods of formulating flours and incorporating them into food compositions are also disclosed. The invention also comprises flours with significant digestible protein and unique dietary fiber content and associated water binding, texturizing, and healthy oil delivery attributes. Novel methods of oil and fat replacement using flours of the invention are also disclosed. Flours of the invention can be manufactured from edible and inedible heterotrophic fermentation feedstocks, including corn starch, sugar cane, glycerol, and depolymerized cellulose.

Microalgal flour

Provided herein are surface treating compositions for imparting beneficial surface properties to substrates. The compositions can be prepared by reacting a bio-based polyol with an isocyanate and an ionogenic molecule. The compositions can be used to treat a variety of substrates to provide enhanced properties to a surface of the substrate. Also provided are methods for the chemical modification of triglycerides and fatty acids and use thereof in creating beneficial surface treating compositions.

Polyols, polyurethane dispersions, and uses thereof

Provided herein are high oleic oil compositions. Further provided herein are methods of producing high oleic oil compositions from microorganisms and applications thereof in end products, including, for example, polyols, polyurethane products, personal care products, and food products.

High oleic oil compositions and uses thereof

This disclosure provides a triglyceride oil possessing an extremely low cloud point and low viscosity concomitant with a higher than anticipated saturated fatty acid content, very low polyunsaturated fatty acid content, and low iodine value. While many naturally occurring triglyceride oils possess one or more of these properties, natural triglyceride oils lack one or more of these attributes, thus making them less than ideal in industrial applications, such as lubricants, fuels, or dielectric fluids. The combination of attributes possessed by a triglyceride oil described herein, achieved without the addition of any additives, is unique compared with natural counterparts and as such, can find wide applications in the aforementioned fields.

Triglyceride oil compositions

Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desaturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

Structuring fats and methods of producing structuring fats

The present invention relates to variant thioesterases and their use in plants, e.g., to increase enzymatic activity and to promote increased production of mid-chain length fatty acids (e.g., 8 to 14 carbons) and at desired ratios. Further disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods described herein.

Variant thioesterases and methods of use

The invention features plant acyl-ACP thioesterase genes of the FatB class and proteins encoded by these genes. The genes are useful for constructing recombinant host cells having altered fatty acid profiles. Oleaginous microalga host cells with the new genes or previously identified FatB genes are disclosed. The microalgae cells produce triglycerides with useful fatty acid profiles.

Thioesterases and cells for production of tailored oils

Growing community of inventors

La Jolla, CA, United States of America

Scott Franklin