The Nisshin Oillio Group, Ltd.

Novozymes A/s

Satoshi Negishi

Hidetaka Uehara

Junko Suzuki

Shin Arimoto

Tomomi Suganuma

Yoshie Yamauchi

Yuri Arai

Tadashiro Hirose

Akira Akahane

Kinya Tsuchiya

Hirofumi Haruna

Yoshiyuki Hatano

Takashi Wada

Yuka Kikuchi

Hans Christian Holm

Yuko Toyama

Hideaki Maki

Tamami Manabe

Toshihisa Suzuki

Chika Sakurai

Isamu Takahashi

Hiroyuki Itagaki

Yumi Kawanobe

Hitoshi Itou

Kunihiko Tokunaga

Maiko Ishizuka

The present invention provides a lipase powder composition which comprises a filter aid(s) and a product obtained by pulverizing asp.-derived lipase immobilized to a silica carrier(s) into the average particle diameter of 1 &#x3bc;m or more and less than 300 &#x3bc;m. This lipase powder composition improves the lipase activity and operability and, therefore, can be suitably used in the methods for exchanging esters of fats and oils and for esterification.

Lipase powder compositions

Object: To provide a chocolate or chocolate-like food capable of inhibiting occurrence of low-temperature bloom. Means for solving problem: A chocolate or chocolate-like food of the present invention comprises an oil and fat, wherein the oil and fat comprises 50 to 85% by weight of triglyceride having an oleic acid at position 2 and saturated fatty acids having 16 or more carbon atoms at positions 1 and 3 (XOX type triglyceride); and 5 to 18% by weight of triglyceride in which two oleic acids are bound and one saturated fatty acid having 16 or more carbon atoms is bound (XO2 type triglyceride). According to such a chocolate or chocolate-like food, the low temperature bloom can be inhibited.

Chocolate or chocolate-like food and method of producing the same

The present invention provides a method for performing regeneration of a decolorization capacity of waste clay that has been used for purification of fats and oils, and production of a thermally recyclable compound as a biofuel from oily ingredients in the waste clay at the same time in a convenient manner. That is, a method for producing purified fats and oils of the invention includes: a method for producing regenerated clay including the steps of mixing waste clay that has been used for purification of fats and oils, lower alcohol, and an acidic catalyst; and performing extraction of oily ingredients from the waste clay, and an esterification reaction between the fats and oils and/or a free fatty acid in the oily ingredients and the lower alcohol at the same time so as to regenerate a decolorization capacity of the waste clay; regenerated clay that is produced by the method for producing the regenerated clay; and a process of decolorizing the fats and oils using the regenerated clay.

Method for producing regenerated clay, regenerated clay, and method for producing purified fats and oils

The present invention relates to a process for preparing a hard butter, which comprises conducting transesterification between one or more selected from the group consisting of straight-chain saturated C16-22 fatty acids and lower alcohol esters thereof and a triglyceride having oleoyl group and/or linoleoyl group at the 2-position with an action of a granulated powder lipase comprising a lipase derived fromand/orand a soybean powder, and removing the granulated powder lipase after the transesterification. The process is industrially suitable process for preparing a hard butter, which have excellent properties as a cacao butter equivalent.

Process for preparing hard butter

The present invention discloses a powdery lipase preparation which is a granulated material comprising a lipase derived fromand/or a lipase derived fromand a soybean powder having a fat content of 5 mass % or more. A lipase activity is improved by using this powdery lipase preparation.

Powdery lipase preparation, method for producing the same and use thereof

The present invention provides a method for producing a ground product of a lipase powder composition comprising an immobilized lipase in which a lipase derived fromsp. is immobilized to a silica carrier and a hydrophilic powder other than a silica carrier, the method comprising the steps of wetting the hydrophilic powder with oil; separating the hydrophilic powder wetted with oil from the oil to obtain an oil-containing hydrophilic powder; mixing the oil-containing hydrophilic powder and the immobilized lipase at a ratio of 0.45:1 to 0.85:1 based on mass of the hydrophilic powder and the immobilized lipase before oil wetting; and grinding the obtained mixture, the steps being conducted in the above order.

Method for producing lipase powder compositions

There is provided a lipase powder which is a granulated material comprising a lipase and at least one member selected from the group consisting of fatty acids having 8 to 12 carbon atoms, alcohol esters thereof, and a mixture thereof. This lipase powder has an increased lipase activity.

Lipase powder, method for manufacture thereof, and use thereof

Disclosed is an oil-and-fat composition suitable for the coating with a tempered chocolate, particularly suitable for the enrobing with the chocolate. Also disclosed is a low-fat tempered chocolate suitable for coating, particularly enrobing. Specifically disclosed is an oil-and-fat composition which has a content of an oleoyldipalmitin (P2O) component of 70 to 85% by mass, which contains a symmetric triglyceride 1,3-dipalmitoyl-2-oleoylglycerin (POP) in the oleoyldipalmitin (P2O) component at a ratio of the amount of 1,3-dipalmitoyl-2-oleoylglycerin (POP) to the amount of the oleoyldipalmitin (P2O) component [(POP)/(P2O)] of 0.95 or more by mass, and which has a content of tripalmitin (PPP) of 2 to 6% by mass. The oil-and-fat composition is added to a tempered chocolate.

Oil-and-fat composition, process for production of the composition, chocolate, and process for production of the chocolate

The present invention discloses a method of producing triglycerides rich in XOX fat and/or XLX fat, which comprises the steps of heating and dissolving triglycerides (XOX fat and/or XLX fat) which comprise 20 to 60 mass % of a triglyceride having a saturated fatty acid residue on each of the first and third position and an oleoyl group and/or a linoleoyl group on the second position in total triglycerides in the presence of 1 to 30 mass % of a fatty acid lower alkyl ester; and then cooling the mixture to precipitate crystals and conducting solid-liquid separation. This method is a more efficient and industrially suitable fractionation and production method of fats and oils which are rich in a triglyceride (XOX fat and/or XLX fat) having a saturated fatty acid residue on each of the first and third position and an oleoyl group and/or a linoleoyl group on the second position.

Fractionation method of 1,3-disaturated-2-unsaturated triglyceride

A lipase powder which is a granulated substance containing a lipase and a solid content of animal milk, a lipase composition wherein said lipase powder is immersed or impregnated in fatty oil, and a method for producing the lipase powder which comprises the step of adding animal milk or cream derived from the animal milk to an aqueous solution containing a lipase, and the step of spray-drying, freeze-drying or solvent-precipitating the mixture thereof are provided. According to the present invention, a lipase powder of which lipase activity and stability are improved can be provided.

Lipase powder, methods for producing the same and use thereof

The present invention discloses a method of recovering lipase activity which comprises the steps of using a lipase derived fromsp. and immobilized on a carrier, or a lipase powder composition which comprises a filter aid and the lipase derived fromsp. and immobilized on a carrier which is crushed into the average particle size of 1 &#x3bc;m or larger and smaller than 300 &#x3bc;m in an esterification or transesterification reaction; and washing said lipase or lipase powder composition with triacylglycerol. According to this method, the decreased lipase activity can be effectively recovered.

Method of recovering lipase activity

There is provided a lipase composition comprising (a) a powdered lipase which is a lipase derived fromsp. or a powdered lipase which is a lipase derived fromsp. and (b) a powdered lipase selected from the group consisting of a powdered lipase which is a lipase derived fromsp., a powdered lipase which is a lipase derived fromsp. and a powdered lipase which is a lipase derived fromsp. When using this lipase composition, a compound(s) having at least one alcoholic hydroxyl group in the molecule can be effectively esterified with a carboxylic acid(s).

Lipase powder composition and a process for preparing an esterified compound by using the same

There are provided an intermolecular compound of (a) di-saturated medium chain fatty acids mono-saturated long chain fatty acid triglyceride and (b) 1,3-di-saturated long chain fatty acids 2-mono-unsaturated long chain fatty acid triglyceride, of which a long spacing value by X-ray diffraction is 65 &#x212b; or more, and foods containing the intermolecular compound. The intermolecular compound can be used as a part of fats and oils that constitute foods. Due to formation of the intermolecular compound, the fats and oils containing large amounts of symmetric triglycerides such as cocoa butter and those containing medium chain fatty acids do not form separate crystals and, therefore, can keep smooth texture and prevent blooming.

Intermolecular compounds of fatty acid triglycerides

A method for producing symmetric triglycerides, which comprises the steps of reacting a medium chain fatty acid triglyceride with a long chain fatty acid triglyceride in the presence of an enzyme or a chemical catalyst to conduct random transesterification reaction and thereby to obtain a reaction product containing a triglyceride composed of a medium chain fatty acid and a long chain fatty acid as the constituting fatty acids in the step of the first reaction; transesterifying the reaction product with an alcohol monoester of the medium chain fatty acid in the presence of an sn-1,3-position specific enzyme in the step of the second reaction and then taking the alcohol monoester of the medium chain fatty acid and the alcohol monoester of the long chain fatty acid from the reaction product obtained in the step of the second reaction to obtain the symmetric triglyceride composed of the medium chain fatty acids at the sn-1, 3 positions and the long chain fatty acid at the sn-2 position. According to this method, highly pure symmetric triglycerides comprising a medium-chain fatty acid at sn-1 and 3 positions and a long chain fatty acid at sn-2 position can be industrially efficiently produced.

Method for producing symmetric triglycerides

An easy and inexpensive process by which a concentrate of a given unsaturated fatty acid can be obtained from a mixture which has conventionally been difficult to concentrate. The process, which is for producing a concentrate of a desired isomer (a) from a mixture (A) selected from the group consisting of a mixture comprising at least two isomers of a Cor higher conjugated unsaturated fatty acid and a mixture comprising at least two cis-isomers of a Cor higher unsaturated fatty acid having a cis-double bond, is characterized by comprising: a step in which the mixture (A) is mixed with at least one Csaturated fatty acid (B) to obtain a mixture solution containing the isomer (a) dissolved therein; a crystallization step in which either crystals rich in the isomer (a) or crystals poor in the isomer (a) are precipitated from the mixture solution; and a solid-liquid separation step for obtaining the crystals rich in the isomer (a) or for obtaining a solution rich in the isomer (a) by removing the crystals poor in the isomer (a).

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Yokosuka, Japan

Satoshi Negishi