National Institute of Advanced Industrial Science and Technology

Satoru Ohgiya

Takehiko Sahara

Kosei Kawasaki

Yoshihiro Ohmiya

Yoichi Kamagata

Kazuhiro Fujimori

Chun Wu

Sakae Tsuda

Isao Yumoto

Yuji Yokota

Tamotsu Hoshino

Hidemasa Kondo

Takako Goda

Yousuke Morita

Takashi Akamatsu

Yasushi Ohyama

Taisuke Seike

Yosuke Kobayashi

Hisataka Taguchi

Michiko Kiriaki

The present invention provides information on a mutant xylose isomerase gene and a mutant protein with which it is possible to impart high xylose metabolic capacity to budding yeast. Also provided is a yeast strain having the mutant xylose isomerase gene. Additionally provided is an efficient method for producing a useful substance using the yeast strain. The present invention provides mutant--derived xylose isomerase (CpXI) having high xylose metabolic activity, the CpXI comprising an amino acid sequence corresponding to an amino acid sequence in which the number 63 threonine of SEQ ID NO: 11 of CpXI is substituted by isoleucine, leucine, glycine, or histidine and/or the number 162 valine is substituted by alanine. Also provided are: a transformed yeast having high ethanol productivity, the transformed yeast being obtained by transformation using a mutant CpXI gene that comprises a codon mutation corresponding to a codon mutation in which the number 63 threonine of SEQ ID NO: 11 in a CpXI gene optimized for the preferred codon of budding yeast is substituted by isoleucine, leucine, glycine, or histidine and/or the number 162 valine is substituted by alanine; and a method for producing ethanol using the transformed yeast.

Mutant xylose-metabolizing enzyme and use thereof

The present invention pertains to: mutant genes having a mutation such as a base substitution in the MTH1, GRR1 and/or CDC19 coding regions thereof; mutant proteins coded by said mutant genes; an upstream region of the GRR1 coding region having a mutation such as a base substitution; a yeast such ashaving said upstream region; and a method for producing a substance such as ethanol by using said yeast.

Yeast having enhanced xylose fermentation ability, and use therefor

The present invention relates to a method for producingluciferase labeled with hydrophilic biotin, characterized in that a biotin reagent containing a polyalkylene glycol structure as a spacer is reacted withluciferase, and biotin-labeledluciferase wherein a sugar chain inluciferase has been biotinylated.

[object Object]

An object of the present invention is to provide a mutant luciferase having luciferase activity with an altered emission spectrum. A specific amino acid residue(s) is substituted in a luciferase derived fromand then the resulting mutant luciferase having luciferase activity with an emission spectrum differing from that of the wild-type luciferase is screened for.

Mutant luciferase

The present invention provides antifreeze proteins produced by a basidiomycete. The antifreeze protein has a high antifreeze activity such as a thermal hysteresis activity or an ice-recrystallization inhibition activity.

Antifreeze proteins from basidiomy cetes

The present invention provides a DNA fragment having a cold-inducible promoter function of yeast, which has high activity in a low temperature range, by identifying a DNA fragment, which exists in a non-translation region located upstream of the 5'-terminal side of a gene selected from the group consisting of cold-inducible genes of, and has a cold-inducible promoter function.

Yeast-origin promoter and vector and expression system using the same

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Hokkaido, Japan

Satoru Ohgiya