The General Hospital Corporation

Harvard College

University of California

Virus Research Institute

The Shriner's Hospital for Crippled Children

Samuel I Miller, Iii

John J Mekalanos

Michael E Selsted

Andre J Ouellette

Yichen Lu

Kevin Killeen

A vector capable of integrating into the chromosome of Salmonella including a first DNA sequence encoding a heterologous protein, a second DNA sequence encoding a marker, and a third DNA sequence encoding a phoP regulatory region regulated gene product necessary for virulence, the third DNA sequence being mutationally inactivated.

Salmonella virulence genes

A modified Salmonella, wherein the wild type pag gene such as pagC has been replaced by a gene encoding a truncated pag gene such as pagC gene fused to a heterologous DNA segment, is disclosed. This modification significantly attenuates the virulence of the Salmonella. In addition, immune reaction to the portion of the fusion protein encoded by the heterologous gene can be generated. Uses of the vector, including its use in a drug screen are also disclosed.

Salmonella vectors encoding truncated pag fusion protein, method of

A vector capable of integrating into the chromosome of Salmonella including a first DNA sequence encoding a heterologous protein, a second DNA sequence encoding a marker, and a third DNA sequence encoding a phoP regulatory region regulated gene product necessary for virulence, the third DNA sequence being mutationally inactivated

A bacterial cell the virulence of which is attentuated by a first mutation in a PhoP regulon and a second mutation in an aromatic amino acid synthetic gene and bacterial cells the virulence of which is attenuated by a mutation in one or more PhoP-activated genes or one or more PhoP-repressed genes.

Salmonella vaccines

The present invention provides substantially purified cryptdin peptides having a consensus amino acid sequence as follows:

Growing community of inventors

Brookline, MA, United States of America

Samuel I Miller, Iii