Wisconsin Alumni Research Foundation

Samira M Azarin

Sean Paul Palecek

Eric V Shusta

Ethan S Lippmann

Xiaojun Lance Lian

Timothy Joseph Kamp

Jianhua Zhang

In one embodiment, the present invention is a method of creating a fully-human blood-brain barrier (BBB) model, comprising the steps of (a) obtaining a mixture of neural cells and brain microvascular endothelial cells (BMECs), wherein the neural cells and BMECs that comprise the mixture were produced from the differentiation of human pluripotent stem cells (hPSCs); (b) purifying BMECs from the mixture of neural cells and BMECs of step (a); and (c) co-culturing the purified BMECs with a cell type selected from the group consisting of pericytes, astrocytes and differentiated neural progenitor cells (NPCs), wherein a blood brain barrier model is created.

Retinoic acid enhanced human stem cell derived blood brain barrier model

A model blood brain barrier obtained from hPSCs is disclosed.

Human blood-brain barrier endothelial cells derived from pluripotent stem cells and blood-brain barrier model thereof

Methods for culturing the pluripotent stem cells to undergo epithelial-to-mesenchymal transition and for generating high-yield, high-purity cardiomyocyte cultures from pluripotent stem cells are described. Pluripotent stem cells are cultured on a support with an overlaid matrix and, optionally, exposed to one or more factors to induce epithelial-to-mesenchymal transition and cardiogenesis.

Growing community of inventors

Chicago, IL, United States of America

Samira M Azarin