Jiangnan University

Sailan Zhang

Li Zhou

Zhongmei Liu

Wenjing Cui

Zhemin Zhou

Junling Guo

Ting Li

Zhongyi Cheng

Dezhi Chen

The disclosure discloses a mutant of nitrile hydratase derived from, and belongs to the technical field of enzyme engineering. The nitrile hydratase mutantNhase-A20V provided by the disclosure has a half-life of about 10 min at 70° C., which does not change much compared with the thermal stability of the wild enzyme. The specific enzyme activity of the mutantNhase-A20V is 128% of that of the wild enzyme. At the same time, the mutant also has better tolerance to a substrate and a product, and the final yield of nicotinamide produced by whole-cell catalysis reaches 598 g/L. Therefore, the nitrile hydratase mutantNhase-A20V provided by the disclosure has good enzymatic properties and is beneficial to future industrial production.

Mutant of nitrile hydratase derived from

Growing community of inventors

Wuxi, China

Sailan Zhang