Toyo Boseki Kabushiki Kaisha

Daiichi Seiyaku Co., Ltd.

Ryotaro Kotani

Setsuo Kobayashi

Kazuo Beppu

Shigeki Otawara

Tsuneo Unuma

Hiroshi Shimizu

Tadao Suzuki

Satoshi Ogawa

This invention discloses a process for producing tissue plasminogen activator. The process comprises cultivating Escherichia coli, yeast, or mammalian cells into which the vector having the gene for human tissue plasminogen activator (tPA) has been introduced by the recombinant DNA technology. In the cultivation or purification process, a specific antiplasmin agent, which is one of the protease inhibitors, is added to the medium and/or the buffer solutions used for purification, so as to prevent the conversion of single-chain form tPA into double-chain form tPA by a protease which is present in the medium.

Process for producing tissue plasminogen activator

There is provided a stable enzyme composition comprising glutathione peroxidase and at least one stabilizer compound selected from the group consisting of pentoses, hexoses, penthahydric sugar alcohols, hexahydric sugar alcohols and disaccharides.

Stable enzyme composition

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Moriyama, Japan

Ryotaro Kotani