Ajinomoto Co., Ltd.

Ryosuke Yumioka

Daisuke Ejima

Tsutomu Arakawa

Yuichi Suzuki

Naoya Yamato

Hiroji Ishii

Masako Koyama

Tatsuru Tabobashi

The present invention provides a method for producing a protein which has a restored native higher-order structure by bringing a protein which has lost its native higher-order structure into contact at pH 6.5 to 9.0 with a 1 to 3% aqueous solution of a specific surfactant, such as lauroylglutamic acid to obtain a solubilized solution of the protein; and then adding the solubilized solution to a buffer with pH 6.5 to 9.0 containing arginine or an arginine derivative at a concentration of 0.1 to 1.2 M to lower the concentration of the specific surfactant, such as lauroylglutamic acid, in the obtained mixture solution down to 0.02 to 0.275%. According to the present invention, it is possible to easily restore the native higher-order structure of a protein while smoothly removing the surfactant from the protein.

Protein refolding method

The present invention provides a method of purifying an antibody by protein A affinity chromatography. More specifically, the present invention provides a technique relating to an elution buffer solution which provides a good antibody recovery rate without denaturation.

Purification method which prevents denaturation of an antibody

A cosmetic composition for skin and/or hair, which comprises at least one kind of substance selected from the group consisting of an N,N-di-acylarginine, for example, a compound represented by the following general formula (I): wherein Rand Reach independently represents a straight or branched-chain alkyl group having 1 to 21 carbon atoms or a straight or branched-chain alkenyl group having 2 to 21 carbon atoms. The cosmetic composition imparts much moistness and superior conditioning effects such as body or elasticity to hair, and to skin, said composition does not impart tackiness and blocked feeling, whilst imparts superior feeling of use such as moistness.

Cosmetic composition

A method to more quantitatively recover a peak of a protein and associated aggregates, hydrophobic proteins, or hydrophobic peptides which are typically difficult to recover and detect as a peak, using a commercially available gel filtration chromatography column and a mobile phase is described. More specifically, a compound, such as arginine, which may be added to the mobile phase, thereby eliminating the unnecessary interaction that occurs between the proteins, peptides, or aggregates and the column stationary phase, is described.

Method for improving recovery yield in protein purification with gel filtration chromatography

The present invention provides a production method of pyrrolidone carboxylic acid or a salt thereof, which includes bringing glutamic acid or a salt thereof into contact with high-temperature high-pressure water having a temperature of above 100&#xb0; C. and not higher than 300&#xb0; C., and a pressure higher than the vapor pressure of water at this temperature. The present invention affords efficient production and supply of pyrrolidone carboxylic acid or a salt thereof from glutamic acid or a salt there.

Production method of pyrrolidone carboxylic acid and salt thereof

An N-long-chain acyl neutral amino acid ester containing a straight-chain or branched-chain, saturated or unsaturated acyl group having from 6 to 22 carbon atoms, where the hydrocarbon group of the alcohol constituting the ester is a straight-chain or branched-chain, saturated or unsaturated hydrocarbon group having from 1 to 10 carbon atoms disclosed herein is an oily material suitable for cosmetic compositions, which provide an excellent feeling upon use and also an excellent hair conditioning effect to a cosmetic composition containing the same. An ultraviolet ray-absorbing composition containing the N-long-chain acyl neutral amino acid ester or/and an N-long-chain acyl acidic amino acid diester is provided, which also has excellent feel. An inorganic pigment composition containing the N-long-chain acyl neutral amino acid ester or/and the above-mentioned N-long-chain acyl acidic amino acid diester is also provided, which has excellent dispersion (stability) of the inorganic pigment, is less irritating to the skin, has excellent feel upon use and is not sticky.

Growing community of inventors

Kawasaki, Japan

Ryosuke Yumioka